Antagonistic effect of live probiotics on growth of Candida albicans on denture base acrylic resin surface

Abstract

Denture stomatitis is often associated with microbial colonization of the acrylic base of the prosthesis, favored by poor hygiene and high level of Candida spp. in the oral cavity. Candida albicans is the fungal species most commonly associated with this oral pathology, usually treated with antifungal agents. The indiscriminate use of these drugs can promote resistance of these strains and side effects to individuals. Thus, it is desirable to promote health through natural or alternative therapies such as the use of probiotic microorganisms. They are available as dietary supplements and are commonly added to foods, including a wide variety of microorganisms, such as Lactobacillus and Bifidobacterium. Probiotics are known for their ability to inhibit the growth of pathogens by competitive, antagonistic, and immune actions. Studies have shown promising results of probiotics effects in the oral cavity, however, literature is scarce about dental materials and probiotics. The aim of this study is to evaluate in vitro, the influence of six different probiotics used in food products, in inhibiting biofilm formation in thermally activated acrylic resin. 80 specimens of acrylic resin will be divided into 6 probiotics groups (n = 10), placebo group (negative control - artificial saliva), and positive control (0.12% chlorhexidine). C. albicans biofilms will be formed on acrylic resin surfaces in the presence of probiotics. For quantification biofilm, the number of culturable cells on the specimens will be evaluated by colony-forming units counting (CFUs) and XTT reduction assay. The data will be submitted to statistical analysis (SPSS 20.0, Armonk, NY, USA).