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Development of biosensors based on zein, hemoglobin and carbon black for hydrogen peroxide detection

Grant number: 16/18270-2
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): October 01, 2016
Effective date (End): September 30, 2017
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
Principal researcher:Bruno Campos Janegitz
Grantee:Tamires dos Santos Pereira
Home Institution: Centro de Ciências Agrárias (CCA). Universidade Federal de São Carlos (UFSCAR). Araras , SP, Brazil


In recent years, the search for new materials has gained attention of many researchers, become it attractive to develop nanostructured composite capable for being applied as devices for the detection of species of interest. Therefore, biosensors stand out, since they comprise biomolecules and other nanometric compounds, which confer advantageous qualities such as high sensitivity, selectivity, low analysis time and reduced costs. The polymer characteristics such as zein are high starch concentration, biocompatibility, biodegradability, low toxicity and ability to form stable dispersions in aqueous solutions corroborate that it can present the support function of nanomaterials and biological materials. Carbon black is a material already used in electroanalytical devices because it has interesting electrochemical properties. Since hemoglobin is a protein biomolecule it can be used for obtaining biosensors, once can assign features to improve considerably the performance of these devices. Accordingly, hemoglobin is has the heme group, which provides high selectivity and sensitivity to hydrogen peroxide. Thus, this project seeks proposals for new biosensor architectures using hemoglobin, carbon black, and zein. Moreover, we intend apply the proposed biosensor in the determination hydrogen peroxide in biological and cosmetic samples.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PEREIRA, TAMIRES DOS SANTOS; MAURUTO DE OLIVEIRA, GABRIELA C.; SANTOS, FABRICIO APARECIDO; RAYMUNDO-PEREIRA, PAULO A.; OLIVEIRA, JR., OSVALDO N.; JANEGITZ, BRUNO CAMPOS. Use of zein microspheres to anchor carbon black and hemoglobin in electrochemical biosensors to detect hydrogen peroxide in cosmetic products, food and biological fluids. Talanta, v. 194, p. 737-744, . (15/19099-2, 16/01919-6, 17/17572-8, 16/04743-6, 16/18270-2, 13/14262-7)

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