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Detection of myxosporean in oligochaetes from fish farms of hybrid pintado (Pseudoplatystoma corruscans x Pseudoplatystoma reticulatum) in Mato Grosso do Sul state, Brazil

Grant number: 15/18807-3
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): September 01, 2016
Effective date (End): October 31, 2020
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
Principal researcher:Antonio Augusto Mendes Maia
Grantee:Tiago Milanin
Home Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
Associated scholarship(s):17/11823-9 - Development of an eDNA sample analysis workflow for estimation of myxozoan (Cnidaria) parasite biodiversity in river water samples, BE.EP.PD


The fish consumption has increased significantly in Brazil, however, mass production has been followed by pathogen agents. Among these, the phylum Myxozoa Grassé, 1970, stands out among the most important ones. Since 1984, in researches with Myxobolus cerebralis and later with other species, it is known that these parasites alternate between the host fish (myxospore stage) and an invertebrate host (actinospore stage), different stages of the same parasite. Up to the present moment, no more than 47 species of myxosporean had their life cycles elucidated. A great part of the diagnostic methods used to myxosporean are held at myxospore stage, through classical microscopy and histopathology tests that require great work and time. For actinospore stage, the diagnosis is more difficult, since, even nowadays, it depends on the observation of actinospores released by Oligochaetes under laboratory conditions. Despite the increasing production of fish, works with Brazilian fish myxosporean are still limited.Thus, the development of faster diagnostic methods capable of detecting the parasite in its early stages of development in invertebrate host is necessary, and it coud be an alternative for the indication of prophylactic measurements and thus reduce the spread of infection to fish . In this study, conventional PCR and real-time PCR (qPCR) will be applied in myxosporean detection in hybrid pintado from a fish farm , an important fish for Brazilian economy and, in identifying and quantifying these parasites, in oligochaetes possibly involved in the life cycle of the parasite.

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Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SOUSA, FABRICIO B.; MILANIN, TIAGO; MORANDINI, ANDRE C.; ESPINOZA, LUIS L.; FLORES-GONZALES, ANAI; GOMES, ANA L. S.; MATOSO, DANIELE A.; MATHEWS, PATRICK D.. Molecular diagnostic based on 18S rDNA and supplemental taxonomic data of the cnidarian coelozoic Ceratomyxa (Cnidaria, Myxosporea) and comments on the intraspecific morphological variation. ZOOSYSTEMATICS AND EVOLUTION, v. 97, n. 2, p. 307-314, . (15/18807-3, 15/21007-9, 18/20482-3)
MILANIN, TIAGO; ATKINSON, STEPHEN D.; SILVA, MARCIA R. M.; ALVES, ROBERTO G.; TAVARES, LUIZ EDUARDO R.; RIBEIRO, AMANDA M.; MAIA, ANTONIO A. M.. Occurrence of two novel actinospore types (Cnidaria: Myxozoa) in fish farms in Mato Grosso do Sul state, Brazil. Parasitology Research, v. 117, n. 6, p. 1757-1764, . (15/18807-3)
MATHEWS, PATRICK D.; MERTINS, OMAR; MILANIN, TIAGO; ESPINOZA, LUIS L.; FLORES-GONZALES, ANAI P.; AUDEBERT, FABIENNE; MORANDINI, ANDRE C.. Molecular Phylogeny and taxonomy of a new Myxobolus species from the endangered ornamental fish, Otocinclus cocama endemic to Peru: A host-parasite coextinction approach. Acta Tropica, v. 210, . (16/13368-4, 15/18807-3, 18/20482-3, 15/23948-5, 15/21007-9)
NALDONI, JULIANA; PEREIRA, JOSE O. L.; MILANIN, TIAGO; ADRIANO, EDSON A.; SILVA, MARCIA R. M.; MAIA, ANTONIO A. M.. Taxonomy, phylogeny and host-parasite interaction of two novel Myxobolus species infecting Brycon orthotaenia from the Sao Francisco River, Brazil. Parasitology International, v. 76, . (15/18807-3, 14/22700-7)
MILANIN, TIAGO; MATHEWS, PATRICK D.; MERTINS, OMAR; TAVARES, LUIZ E. R.; SILVA, MARCIA R. M.; MAIA, ANTONIO A. M.. Molecular phylogeny of the gill parasite Henneguya (Myxosporea: Myxobolidae) infecting Astyanax lacustris (Teleostei: Characidae) from fish farm in Brazil. Microbial Pathogenesis, v. 123, p. 372-376, . (13/14656-5, 15/23948-5, 15/18807-3)
MILANIN, TIAGO; MATHEWS, PATRICK D.; MORANDINI, ANDRE C.; MERTINS, OMAR; AUDEBERT, FABIENNE; PEREIRA, JOSE O. L.; MAIA, ANTONIO A. M.. Morphostructural data and phylogenetic relationships of a new cnidarian myxosporean infecting spleen of an economic and ecological important bryconid fish from Brazil. Microbial Pathogenesis, v. 150, . (15/21007-9, 19/13147-6, 18/20482-3, 15/18807-3)

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