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Column-switching liquid chromatography-Tandem mass spectrometry for determination of endocannabinoids and cannabinoids in biological samples from patients with Parkinson's Disease

Grant number: 16/13639-8
Support type:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): October 01, 2016
Effective date (End): January 31, 2019
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
Principal researcher:Maria Eugênia Queiroz Nassur
Grantee:Camila Marchioni
Home Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil


Endocannabinoids (eCBs) are neurotransmitters derived from arachidonic acid. eCBs bind to cannabinoid receptors and participate in several brain functions, like motor functions. Alterations in the endocannabinoid system in patients with Parkinson´s Disease (PD) have been evaluated by determination of anandamide (AEA) and 2-arachidonoyl glycerol (2AG), two major eCBs, in biological samples. Recently, cannabidiol (CBD), a derivative of Cannabis sativa, has been evaluated for the treatment of PD in patients with psychotic symptoms. Liquid chromatography-Tandem mass spectrometry is considered the reference analytical technique to determine eCBS and cannabinoid in biological samples. Column switching LC-MS/MS allows automation of the analysis by hyphenating sample biological preparation (preconcentration of analytes and removal of endogenous components) with the chromatographic system (chromatographic separation and detection), which shortens analysis time, reduces the amount of biological sample and the volume of organic solvents, and increases analytical selectivity and sensitivity. We have developed the online column switching liquid chromatography Tandem mass spectrometry (UHPLC-MS/MS) method to determine AEA and 2AG in plasma and cerebrospinal fluid samples from patients with PD. Our future aim is to develop an online column switching UHPLC-MS/MS method that uses a monolithic MIP column in the first dimension and reverse phase in the second dimension to determine CBD and delta 9-THC in plasma samples from patients with PD being treated with CBD. Changes in the endocannabinoid system (AEA and 2AG) of PD patients will be evaluated, to find out whether the use of cannabidiol use alters the levels of these substances. The administered CBD doses will be correlated with CBD and delta 9-THC plasma concentrations in patients with PD. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MARCHIONI, CAMILA; DONIZETI DE SOUZA, ISRAEL; FERNANDES GRECCO, CAROLINE; ALEXANDRE CRIPPA, JOSE; TUMAS, VITOR; EUGENIA COSTA QUEIROZ, MARIA. A column switching ultrahigh-performance liquid chromatography-tandem mass spectrometry method to determine anandamide and 2-arachidonoylglycerol in plasma samples. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v. 409, n. 14, p. 3587-3596, . (15/07619-1, 16/13639-8)
MARCHIONI, CAMILA; DE SOUZA, ISRAEL DONIZETI; ACQUARO JUNIOR, VINICIUS RICARDO; DE SOUZA CRIPPA, JOSE ALEXANDRE; TUMAS, VITOR; COSTA QUEIROZ, MARIA EUGENIA. Recent advances in LC-MS/MS methods to determine endocannabinoids in biological samples: Application in neurodegenerative diseases. Analytica Chimica Acta, v. 1044, p. 12-28, . (17/02147-0, 15/07619-1, 16/13639-8)
MARCHIONI, CAMILA; VIEIRA, TATIANA MANZINI; MILLER CROTTI, ANTONIO EDUARDO; CRIPPA, JOSE ALEXANDRE; COSTA QUEIROZ, MARIA EUGENIA. In-tube solid-phase microextraction with a dummy molecularly imprinted monolithic capillary coupled to ultra-performance liquid chromatography-tandem mass spectrometry to determine cannabinoids in plasma samples. Analytica Chimica Acta, v. 1099, p. 145-154, . (17/02147-0, 16/13639-8)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
MARCHIONI, Camila. Determination of endocannabinoids and cannabinoids in biological samples from patients with Parkinson`s disease by ultrahigh-performance liquid chromatography-tandem mass spectrometry. 2019. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC) Ribeirão Preto.

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