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Integrated fractionation processes of Saccharomyces cerevisiae from the brewing industry for the production of high value-added biomolecules

Grant number: 16/08886-6
Support Opportunities:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): August 01, 2016
Effective date (End): April 30, 2020
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal Investigator:Pedro de Oliva Neto
Grantee:Edson Marcelino Alves
Host Institution: Faculdade de Ciências e Letras (FCL-ASSIS). Universidade Estadual Paulista (UNESP). Campus de Assis. Assis , SP, Brazil


Currently, the yeast biomass is an underutilized waste product of brewing industry. Moreover, the use of this biomass can be an economical source for the production of 5'-ribonucleotides, which are high added value by products and have wide applications in pharmaceutical and food industry. Faced with these issues, this project aims to develop a cost and yield effective methodology for the production of 5'-ribonucleotides from the residual yeast biomass from brewery industry. For this, evaluation of the efficiency of individual cell disruption methods (bead mill, autolysis, acid hydrolysis) and integrated cell disruption methods (autolysis followed by bead mill and bead mill followed by acid hydrolysis) will be made aiming to obtain maximum RNA yield. After cell disruption and RNA content extraction, the 5'-ribonucleotides will be produced by enzymatic hydrolysis of RNA using commercial 5'-phosphodiesterase. Finally, a scale up and economic analysis will be performed in order to determine the profitability and financial viability of the methodology developed in this project. The proposed approach indicates an excellent strategy, since a better use of yeast residue from fermentation processes can open up a range of applications and add value to this product, making the industrial sectors more profitable. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
EDSON MARCELINO ALVES; JOYCE FARIA DE SOUZA; PEDRO DE OLIVA NETO. Avanços na tecnologia de autólise de levedura − um novo bioprocesso mais rápido e seguro. Brazilian Journal of Food Technology, v. 24, . (16/08886-6)
EDSON MARCELINO ALVES; JOYCE FARIA DE SOUZA; SZYMON MACIEJA; PEDRO DE OLIVA NETO. Produção de 5'-ribonucleotídeos utilizando 5'-fosfodiesterase da radícula de malte cervejeiro. Brazilian Journal of Food Technology, v. 24, . (16/08886-6)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
ALVES, Edson Marcelino. Fractionation of spent brewer's yeast for high value-added biomolecules production. 2020. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Pesquisa em Bioenergia. Rio Claro Rio Claro.

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Filed patent(s) as a result of this research project

PROCESSO DE AUTÓLISE DE CÉLULAS DE LEVEDURA BR102019008208-9 - Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp) . Pedro de Oliva Neto / EDSON MARCELINO ALVES / JOYCE FARIA DE SOUZA - April 2019, 23