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Establishment of a stable HTLV-1-infected reporter cell line for antiviral screening assays

Grant number: 16/17301-1
Support Opportunities:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): January 08, 2017
Effective date (End): April 07, 2017
Field of knowledge:Health Sciences - Pharmacy
Principal Investigator:Simone Kashima Haddad
Grantee:Daiane Fernanda dos Santos
Supervisor: Willems
Host Institution: Hemocentro de Ribeirão Preto. Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto da USP (HCMRP). Secretaria da Saúde (São Paulo - Estado). Ribeirão Preto , SP, Brazil
Research place: Université de Liège (ULg), Belgium  
Associated to the scholarship:15/11566-0 - Large-scale screening for identification of novel compounds for treatment of HTLV-1 infection and related clinical conditions, BP.DR

Abstract

Human T cell lymphotropic virus type 1 (HTLV-1) was the first human retrovirus to be discovered. Approximately, 10 - 20 million people are infected with HTLV-1 worldwide, and in Brazil, there are about 2.5 million HTLV-1-carriers. The main HTLV-1-associated diseases are the HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP) and the adult T cell leukemia/lymphoma (ATL). Currently, there is no cure and no preventive vaccine for this infection. Also, the therapeutic approaches for the treatment of HAM/TSP have variable effectiveness. Thus, the identification of new compounds with antiviral action, mainly by high content screening assays (HCS), is necessary. Some studies have adopted the construction of reporter cell lines based on the green fluorescent protein (GFP) gene expression under the control of the viral promoter region, since these tools are fast, easy and inexpensive for antiviral drug studies. Therefore, the aim of this project is to construct a HTLV-1-infected reporter cell line which carries the full viral genome and the GFP gene under the control of the long terminal repeat (LTR) promoter (Jurkat-HTLV-1-LTR-GFP) for use in antiviral screening assays. The construction of this reporter cell line will be a powerful strategy for the identification of new antiviral compounds with inhibitory action on HTLV-1 gene expression.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DOS SANTOS, DAIANE FERNANDA; BAIRROS DE PILGER, DENISE REGINA; VANDERMEULEN, CHARLOTTE; KHOURI, RICARDO; MANTOANI, SUSIMAIRE PEDERSOLI; GONCALVES NUNES, PAULO SERGIO; DE ANDRADE, PETERSON; CARVALHO, IVONE; CASSEB, JORGE; TWIZERE, JEAN-CLAUDE; et al. Non-cytotoxic 1,2,3-triazole tethered fused heterocyclic ring derivatives display Tax protein inhibition and impair HTLV-1 infected cells. Bioorganic & Medicinal Chemistry, v. 28, n. 22, . (15/11566-0, 16/17301-1)

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