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Magnetron sputtering deposition of tantalum oxide (Ta2O5) films onto titanium surface for biomedical applications: electrochemical behavior, biocompatibility and microbiologic analysis

Grant number: 16/07269-3
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): October 01, 2016
Effective date (End): September 30, 2019
Field of knowledge:Health Sciences - Dentistry - Dental Clinics
Principal researcher:Valentim Adelino Ricardo Barão
Grantee:Thamara Beline
Home Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil


This study aims to develop tantalum oxide (Ta2O5) films onto commercially-pure titanium (cp-Ti) surface using magnetron sputtering deposition and to evaluate its electrochemical behavior, biocompatibility and biofilm formation. Cp-Ti discs will be divided into groups: machined (surface I - control) and treated with Ta2O5 films (surface II - experimental). In the electrochemical assay, standard tests such as open circuit potential, electrochemical impedance spectroscopy, and potentiodynamic tests will be conducted in simulated body fluid solution (pH 7.4) An appropriate electrical circuit will be used to determine the capacitance (Cdl) and polarization resistance (Rp) of the oxide layer. The Tafel's method will be used to ascertain the corrosion current density (Icorr) and corrosion potential (Ecorr). The passivation current density (Ipass) will be the amount of current in the transition between the active and passive region in the polarization curve. Discs' topography will be characterized by the scanning electron microscopy (SEM), energy dispersive spectroscopy, atomic force microscopy, X-ray photoelectron spectroscopy, X-ray diffraction analysis, perfilometry and surface free energy. For biological tests, murine osteoprogenitor cell line (MC3T3E1) will be used. The structure and cell morphology will be evaluated by SEM and fluorescence microscopy. The quantitative reverse transcription polymerase chain reaction (qRT-PCR) will be used to check the levels of osteogenic genes expression. MTT assay will be used to assess cell viability. Alizarin test will be performed to identify and quantify the mineral deposits in the cells. The immersion of the samples in body fluid will be conducted to test the bioactivity of the surfaces. For microbiological test, the biofilm composed of Streptococcus sanguinis will formed onto the surface of the discs. The log of colony forming units will be determined (log CFU) and structural biofilm analysis will be performed by SEM. Quantitative data will be submitted to the most appropriate statistical analysis with 5% significance level. The number of specimens for each test will be determined after the pilot study.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BELINE, THAMARA; DE ALMEIDA, AMANDA B.; AZEVEDO NETO, NILTON F.; MATOS, ADAIAS O.; RICOMINI-FILHO, ANTONIO P.; SUKOTJO, CORTINO; SMEETS, PAUL J. M.; DA SILVA, JOSE H. D.; NOCIT, JR., FRANCISCO H.; BARAO, VALENTIM A. R. 0-Ta 2 O 5 thin film for implant surface modification triggers superior anti- corrosion performance and cytocompatibility of titanium. Applied Surface Science, v. 520, AUG 1 2020. Web of Science Citations: 0.
BELINE, THAMARA; DA SILVA, JOSE H. D.; MATOS, ADAIAS O.; AZEVEDO NETO, NILTON F.; DE ALMEIDA, AMANDA B.; NOCITI JUNIOR, FRANCISCO H.; LEITE, DOUGLAS M. G.; RANGEL, ELIDIANE CIPRIANO; BARAO, VALENTIM A. R. Tailoring the synthesis of tantalum-based thin films for biomedical application: Characterization and biological response. Materials Science & Engineering C-Materials for Biological Applications, v. 101, p. 111-119, AUG 2019. Web of Science Citations: 0.
BELINE, THAMARA; VECHIATO FILHO, ALJOMAR JOSE; WEE, ALVIN G.; SUKOTJO, CORTINO; DOS SANTOS, DANIELA MICHELINE; BRANDAO, THAIS BIANCA; RICARDO BARAO, VALENTIM ADELINO. Initial investigation of the corrosion stability of craniofacial implants. JOURNAL OF PROSTHETIC DENTISTRY, v. 119, n. 1, p. 185-192, JAN 2018. Web of Science Citations: 0.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
BELINE, Thamara. Magnetron sputtering deposition of tantalum oxide (Ta2O5) films onto titanium surface for biomedical applications : electrochemical behavior, biocompatibility and microbiologic analysis. 2020. 95 f. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba.

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