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Development of analytical methods for the determination of ayahuasca alkaloids in different matrices applying green analytical chemistry strategies

Grant number: 16/06810-2
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): October 01, 2016
Effective date (End): June 30, 2020
Field of knowledge:Health Sciences - Pharmacy - Toxicological Analysis
Principal Investigator:Mauricio Yonamine
Grantee:Gabriela de Oliveira Silveira
Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil


Ayahuasca is an indigenous hallucinogenic beverage used for shamanic and spiritual purposes, the use of which dates from the pre-columbian period in the Amazon basin region. This beverage is usually prepared by decoction of the Psicotria viridis and Banisteriopsis caapi plants. The ²-carboline alkaloids present in B. Caapi vine are responsible for inhibition of the enzyme monoamine oxidase-A (MAO-A), allowing the hallucinogen DMT (dimethyltryptamine), present in the leaves of P. viridis, to reache its active site and to promote the hallucinogenic action in serotonergic receptors. With its widespread use in Brazil, North America and Europe, the ayahuasca tea is of great interest for the scientific community and it is being studied by several Brazilian research groups. In this context, the development of analytical methodologies which will enable the determination of the main alkaloid of this drink in various matrices is indeed very important and it represents a constant demand at the Laboratory of Analytical Toxicology (USP) from researchers of different Brazilian institutions. Besides, our research team believes in the importance of promoting the development of analytical technology in sample treatment which meets the global trend of sustainable development by implementing strategies of 'green analytical chemistry' (GAC): minimizing the consumption of solvents and energy, elimination of derivatizing agents, low cost and ease of implementation. Thus, the aim of this study is to develop and to validate methods of extraction for ayahuasca alkaloids in tea samples, plasma, urine and hair, using methods based on GAC, in order to meet the demand of ayahuasca research groups from various institutions in the country.

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SILVEIRA, GABRIELA DE OLIVEIRA; FONSECA PEGO, ANA MIGUEL; PEREIRA E SILVA, JEFFERSON; YONAMINE, MAURICIO. Green sample preparations for the bioanalysis of drugs of abuse in complex matrices. BIOANALYSIS, v. 11, n. 4, p. 295-312, . (16/06810-2)
SILVEIRA, GABRIELA DE OLIVEIRA; DOS SANTOS, RAFAEL GUIMARAES; LOURENCO, FELIPE REBELLO; ROSSI, GIORDANO NOVAK; HALLAK, JAIME E. C.; YONAMINE, MAURICIO. Stability Evaluation of DMT and Harmala Alkaloids in Ayahuasca Tea Samples. Molecules, v. 25, n. 9, . (16/06810-2, 18/24770-3)
SILVEIRA, GABRIELA DE OLIVEIRA; LOURENCO, FELIPE REBELLO; FONSECA PEGO, ANA MIGUEL; DOS SANTOS, RAFAEL GUIMARAES; ROSSI, GIORDANO NOVAK; HALLAK, JAIME E. C.; YONAMINE, MAURICIO. Essential oil-based dispersive liquid-liquid microextraction for the determination of N,N-dimethyltryptamine and beta-carbolines in human plasma: A novel solvent-free alternative. Talanta, v. 225, . (18/24770-3, 16/06810-2)
SILVEIRA, GABRIELA DE OLIVEIRA; LOURENCO, FELIPE REBELLO; BRUNO, VITOR; YONAMINE, MAURICIO. Fast Hollow Fiber Liquid-Phase Microextraction as a Greener Alternative for the Determination of N,N-Dimethyltryptamine and Harmala Alkaloids in Human Urine. RONTIERS IN CHEMISTR, v. 8, p. 13-pg., . (18/24770-3, 16/06810-2)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SILVEIRA, Gabriela de Oliveira. Development of analytical methods for the determination of ayahuasca alkaloids in different matrices applying green analytical chemistry strategies. 2020. Doctoral Thesis - Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) São Paulo.

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