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Cutaneous leishmaniasis biomarkers candidates search and identification through mass spectrometry

Grant number: 16/11517-2
Support type:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): September 01, 2016
Effective date (End): January 31, 2020
Field of knowledge:Health Sciences - Pharmacy
Principal researcher:Marcos Nogueira Eberlin
Grantee:Fernanda Negrão Silva
Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated scholarship(s):17/22651-4 - Shotgun proteomics for host-pathogen relationship investigation of leishmaniasis in vitro and in vivo, BE.EP.DD

Abstract

Leishmaniasis, caused by protozoa of the genus Leishmania, is a neglected disease in Brazil and worldwide. This proposal aims to apply the MS in the search of biomarkers candidates of Cutaneous Leishmaniasis (CL), the most common clinical form of the disease. The project involves the imaging of proteins and lipids in lesions of infected mice (widely used in experimental models of this disease) referred to the species L. amazonensis and L. major. The MS techniques to be applied, in special MALDI (Matrix Assisted Laser Desorption Ionization) and DESI (Desorption Electrospray Ionization), allows detection of biomolecules with high sensitivity, accuracy and selectivity compared to other classical biomolecules chemical analysis techniques, such as immunohistochemistry, immunofluorescence and immunoblotting. The search of biomarkers candidates with these methodology will give more information about the pathophysiological mechanisms of Leishmania spp., providing excellent prospects in new diagnostic tests and drug development. This proposal will contribute effectively to improve the understanding of the parasite Leishmania spp. biology and pathophysiology. (AU)

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Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PORCARI, ANDREIA M.; NEGRAO, FERNANDA; TRIPODI, GUILHERME LUCAS; PITTA, DENISE ROCHA; CAMPOS, ELISABETE APARECIDA; MONTIS, DOUGLAS MUNHOZ; MARTINS, ALINE M. A.; EBERLIN, MARCOS N.; DERCHAIN, SOPHIE F. M. Molecular Signatures of High-Grade Cervical Lesions. FRONTIERS IN ONCOLOGY, v. 8, APR 12 2018. Web of Science Citations: 1.
NEGRAO, FERNANDA; ABANADES, DANIEL R.; JAEEGER, CAROLINE F.; ROCHA, DANIELE F. O.; BELAZ, KATIA R. A.; GIORGIO, SELMA; EBERLIN, MARCOS N.; ANGOLINI, CELIO F. F. Lipidomic alterations of in vitro macrophage infection by L-infantum and L-amazonensis. MOLECULAR BIOSYSTEMS, v. 13, n. 11, p. 2401-2406, NOV 2017. Web of Science Citations: 3.
NEGRAO, FERNANDA; EBERLIN, MARCOS NOGUEIRA; GIORGIO, SELMA. Proteomic approaches for drug discovery against tegumentary leishmaniasis. BIOMEDICINE & PHARMACOTHERAPY, v. 95, p. 577-582, NOV 2017. Web of Science Citations: 4.
JAEGGER, C. F.; NEGRAO, F.; ASSIS, D. M.; BELAZ, K. R. A.; ANGOLINI, C. F. F.; FERNANDES, A. M. A. P.; SANTOS, V. G.; PIMENTEL, A.; ABANADES, D. R.; GIORGIO, S.; EBERLIN, M. N.; ROCHA, D. F. O. MALDI MS imaging investigation of the host response to visceral leishmaniasis. MOLECULAR BIOSYSTEMS, v. 13, n. 10, p. 1946-1953, OCT 2017. Web of Science Citations: 2.
NEGRAO, FERNANDA; ROCHA, DANIELE F. DE O.; JAEEGER, CAROLINE F.; ROCHA, FRANCISCA J. S.; EBERLIN, MARCOS N.; GIORGIO, SELMA. Murine cutaneous leishmaniasis investigated by MALDI mass spectrometry imaging. MOLECULAR BIOSYSTEMS, v. 13, n. 10, p. 2036-2043, OCT 2017. Web of Science Citations: 5.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.