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Use of bacterial cellulose membrane (Nanoskin) in experimental wounds in sheep

Grant number: 16/12631-3
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): September 01, 2016
Effective date (End): August 31, 2017
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
Principal researcher:Flávia de Almeida Lucas
Grantee:Camila Sabino de Oliveira
Home Institution: Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

The wounds in farm animals occur due to trauma and usually heal by secondary intention. Bacterial cellulose (BC) has been intensively studied and evaluated as tissue repair material and several different products of BC have been successful when applied on the skin healing. With this work, the objective is to make the clinical and thermographic the use of the cellulose membrane (Nanoskin®) in experimentally induced wounds in the metacarpal and metatarsal regions of sheep. Twelve sheep, adult and otherwise healthy are used. With the use of a mold wounds will be made by removing the full thickness skin at the lateral regions of the metacarpal and metatarsal. All wounds will be treated daily with saline solution at 0.9% and maintained with bandage changed daily after dressing. Clinical and thermography evaluation will be carried out immediately before and after the making of the wounds and at 3, 7, and 15dias postoperatively. The data areas will be submitted to analysis of variance split plot with repeated measures and rate of contraction of the wounds to the analysis of variance in a split plot, followed by the Tukey test for multiple comparisons of means. Temperatures obtained (center and edges) with the thermographic evaluation will be analyzed by analysis of variance and Student's t test, using the SAS program (StatisticalAnalysis System).

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