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Interaction between biomembrane models based on Langmuir films and giant unilamellar vesicles (GUVs) with triazine herbicides and detection via SERS spectroscopy.

Grant number: 16/09634-0
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): September 01, 2016
Effective date (End): October 31, 2020
Field of knowledge:Physical Sciences and Mathematics - Physics - Condensed Matter Physics
Principal researcher:Carlos José Leopoldo Constantino
Grantee:Rafael Jesus Gonçalves Rubira
Home Institution: Faculdade de Ciências e Tecnologia (FCT). Universidade Estadual Paulista (UNESP). Campus de Presidente Prudente. Presidente Prudente , SP, Brazil
Associated research grant:13/14262-7 - Nanostructured films from biologically-relevant materials, AP.TEM
Associated scholarship(s):18/04628-8 - Detection of triazine herbicides by SERS using silver nanoparticles synthesised via green chemistry, BE.EP.DR


Phospholipids are a class of materials widely applied as mimetic system of the cell membrane in interaction studies with several analytes. Thus, the use of phospholipids could contribute to a better detection of herbicides and other toxic compounds in sensing applications, considering the susceptibility of living organisms by these compounds. In addition of detection, it may also allow to investigate the effect of these compounds on the wall of cell membrane, having lipids as biomimetic models. This PhD project includes both aspects. Will be investigated the interaction of atrazine, ametrina and prometryn herbicides with the phospholipid dipalmitoyl phosphatidylcholine (DPPC) as a biological membrane model. The interaction of phospholipid with the herbicides will be studied by means of two systems: i) DPPC Langmuir films characterized by surface pressure isotherms vs. mean molecular area (À-A isotherms) and infrared spectroscopy with polarization modulation (PM-IRRAS) and ii) giant unilamellar vesicles (GUVs) of DPPC electroformed and characterized by confocal microscopy/Fluorescence. The other aspect will address the detection of herbicides by means of surface-enhanced Raman scattering spectroscopy (SERS) in colloidal system of Ag nanoparticles (AgNPs) in order to obtain analytical curves from the adsorption of the herbicide onto the surface of AgNPs. In this case the strategy is to dilute the pesticides in AgNPs colloidal systems in the presence and absence of DPPC. The combination of both materials (AgNPs and DPPC) may lead to desirable properties improving the sensitivity for detection, quantitation and interaction of specific chemical species. This project is part of the Thematic Project FAPESP 2013/14262-7 entitled "Nanostructured films of biological interest materials".

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Scientific publications (8)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
RODRIGUES, GUSTAVO H. S.; MIYAZAKI, CELINA M.; RUBIRA, RAFAEL J. G.; CONSTANTINO, CARLOS J. L.; FERREIRA, MARYSTELA. Layer-by-Layer Films of Graphene Nanoplatelets and Gold Nanoparticles for Methyl Parathion Sensing. ACS APPLIED NANO MATERIALS, v. 2, n. 2, p. 1082-1091, . (16/19387-0, 16/09634-0, 14/15093-7, 17/03523-5)
FIGUEIREDO, MARIA L. B.; MARTIN, CIBELY S.; FURINI, LEONARDO N.; RUBIRA, RAFAEL J. G.; BATAGIN-NETO, AUGUSTO; ALESSIO, PRISCILA; CONSTANTINO, CARLOS J. L.. Surface-enhanced Raman scattering for dopamine in Ag colloid: Adsorption mechanism and detection in the presence of interfering species. Applied Surface Science, v. 522, . (17/15019-0, 16/09634-0, 14/50869-6, 17/15219-9)
DE LIMA, LUCAS F.; MACIEL, CRISTIANE C.; FERREIRA, ANDRE L.; RUBIRA, RAFAEL J. G.; CONSTANTINO, CARLOS J. L.; FERREIRA, MARYSTELA. An investigation of the synergistic effect between magnetite nanoparticles and polypyrrole in nanostructured layer-by-layer films. Journal of Applied Polymer Science, v. 138, n. 5, . (16/09634-0, 18/04061-8, 16/19387-0)
ZANASI, GIULIA; RUBIRA, RAFAEL J. G.; FRANCIOSO, ORNELLA; CANAMARES, MARIA VEGA; CONSTANTINO, CARLOS J. L.; SANCHEZ-CORTES, SANTIAGO. Sensing Atrazine Herbicide Degradation Products through Their Interactions with Humic Substances by Surface-Enhanced Raman Scattering. CHEMOSENSORS, v. 9, n. 6, . (16/09634-0, 18/04628-8, 20/05423-0)
OLIVEIRA, MARCELO J. S.; RUBIRA, RAFAEL J. G.; FURINI, LEONARDO N.; BATAGIN-NETO, AUGUSTO; CONSTANTINO, CARLOS J. L.. Detection of thiabendazole fungicide/parasiticide by SERS: Quantitative and mechanism. Applied Surface Science, v. 517, . (13/14262-7, 16/09634-0)
REYES GOMEZ, FAUSTINO; RUBIRA, RAFAEL J. G.; CAMACHO, SABRINA A.; MARTIN, CIBELY S.; DA SILVA, ROBSON R.; CONSTANTINO, CARLOS J. L.; ALESSIO, PRISCILA; OLIVEIRA, JR., OSVALDO N.; RICARDO MEJIA-SALAZAR, J.. Surface Plasmon Resonances in Silver Nanostars. SENSORS, v. 18, n. 11, . (13/01897-4, 16/09634-0, 16/12311-9, 16/06612-6, 13/14262-7)
RUBIRA, RAFAEL J. G.; CAMACHO, SABRINA A.; CONSTANTINO, CARLOS J. L.; SANCHEZ-CORTES, SANTIAGO. Increasing the sensitivity of surface-enhanced Raman scattering detection for s-triazine pesticides by taking advantage of interactions with soil humic substances. Journal of Raman Spectroscopy, v. 53, n. 1, . (18/14692-5, 20/05423-0, 18/04628-8, 16/09634-0, 18/22214-6)
RUBIRA, R. J. G.; FURINI, L. N.; CONSTANTINO, C. J. L.; SANCHEZ-CORTES, S.. SERS detection of prometryn herbicide based on its optimized adsorption on Ag nanoparticles. VIBRATIONAL SPECTROSCOPY, v. 114, . (18/22214-6, 20/05423-0, 18/04628-8, 16/09634-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
RUBIRA, Rafael Jesus Gonçalves. Interaction between biomembrane models based on Langmuir films and giant unilamellar vesicles (GUVs) with triazine herbicides and detection via SERS spectroscopy. 2020. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Ciências e Tecnologia. Presidente Prudente Presidente Prudente.

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