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Selection, Culture and Transcriptome of spermatogonial stem cells of zebrafish, Danio rerio: a functional and genomics approach

Grant number: 16/12101-4
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): August 01, 2016
Effective date (End): December 31, 2018
Field of knowledge:Biological Sciences - Genetics - Animal Genetics
Principal Investigator:Rafael Henrique Nóbrega
Grantee:Lucas Benites Doretto
Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
Associated research grant:14/07620-7 - Spermatogonial stem cell bank to preserve the genotype of endangered and valuable teleost species, AP.JP


Spermatogonial Stem Cells (SSCs) are the only stem cells in the body capable to transmit thegenetic information from species along generations. This feature is due to the potential of these cells to either self-renew or differentiate to give rise to millions of spermatozoa. Recent studies using SSCs have generated promising methods in the aquaculture, such as the SSC transplantation. Thistechnique consists in the isolation of donor SSCs and their transplantation into a recipient testis, where these cells can develop and produce sperm with donor genetic characteristics. In the current project, we propose a SSC bank which in association with transplantation techniques constitutes apowerful tool to preserve endangered and valuable species. Therefore, the aim of this project is to characterize the molecular markers of SSCs using zebrafish (Danio rerio) as a laboratory model; and to apply this knowledge for a native and high economic interest species, such as jundiá (Rhamdia quelen). Then, SSCs from jundiá will be selected based on these molecular markers, andsubmitted to cryopreservation techniques afterwards. To test the viability of the cryopreserved cells, SSCs will be thawed and transplanted into recipient testes. In addition, we aimed to generate vasa::gfp jundiás in order to improve the the visualization of the donor-derived spermatogenesis in the recipient testes. This project will provide the basis to create a SSC bank for several teleost species in order to preserve the genotype of endangered and high economic interest species.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DORETTO, LUCAS B.; BUTZGE, ARNO J.; NAKAJIMA, RAFAEL T.; MARTINEZ, EMANUEL R. M.; DE SOUZA, BEATRIZ MARQUES; RODRIGUES, MAIRA DA SILVA; ROSA, IVANA F.; RICCI, JULIANA M. B.; TOVO-NETO, ALDO; COSTA, DANIEL F.; et al. Gdnf Acts as a Germ Cell-Derived Growth Factor and Regulates the Zebrafish Germ Stem Cell Niche in Autocrine- and Paracrine-Dependent Manners. CELLS, v. 11, n. 8, p. 27-pg., . (17/08274-3, 14/07620-7, 16/12101-4, 20/03569-8)
MARTINEZ-BENGOCHEA, A.; DORETTO, L.; ROSA, I. F.; OLIVEIRA, M. A.; SILVA, C.; SILVA, D. M. Z. A.; SANTOS, G. R.; SANTOS, J. S. F.; AVELAR, M. M.; SILVA, V, L.; et al. Effects of 17 beta-estradiol on early gonadal development and expression of genes implicated in sexual differentiation of a South American teleost, Astyanax altiparanae. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, v. 248, . (14/07620-7, 16/12101-4, 18/10265-5, 14/25313-4)
TOVO-NETO, ALDO; MARTINEZ, EMANUEL R. M.; MELO, ALINE G.; DORETTO, LUCAS B.; BUTZGE, ARNO J.; RODRIGUES, MAIRA S.; NAKAJIMA, RAFAEL T.; HABIBI, HAMID R.; NOBREGA, RAFAEL H.. Cortisol Directly Stimulates Spermatogonial Differentiation, Meiosis, and Spermiogenesis in Zebrafish (Danio rerio) Testicular Explants. BIOMOLECULES, v. 10, n. 3, . (14/07620-7, 14/16280-5, 13/22778-3, 17/15793-7, 16/12101-4, 15/12104-0, 17/09218-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
DORETTO, Lucas Benites. Characterization of spermatogonia stem cell markers and its endocrine and paracrine regulation in zebrafish (Danio rerio). 2018. Master's Dissertation - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Botucatu Botucatu.

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