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Isolation, molecular identification and detection of virulence genes of Yersinia enterocolitica in bulk tank milk samples of properties from Midwest Region of São Paulo State

Grant number: 16/03582-9
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): August 01, 2016
Effective date (End): December 27, 2017
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
Principal Investigator:Simone Baldini Lucheis
Grantee:Amanda Bezerra Bertolini
Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil


There is still a belief, for much of the population, that raw milk as well as tastier is more healthy, nutritious and yet has a lower price, as well as the processed milk has preservatives and chemicals that can affect the health of those who consuming. These arguments are used by people who consume raw milk in various states of Brazil. However, milk and dairy products are great means for the development of pathogenic and spoilage microorganisms, necessitating the need for strict care of milking, processing and storage. Among the various groups of bacteria that may develop in the refrigerated raw milk, there are the psychrotrophic, since multiply at temperatures below 7 °C, and thus represent a high risk for the consumer of cold raw milk. Among these psychrotrophic bacteria, Yersinia enterocolitica, which is an invasive enteropathogen of humans, causes a series of intestinal clinical symptoms and extra-intestinal disorders, ranging from mild gastroenteritis to a mesenteric lymphadenitis, may mimic appendicitis above and, in rare cases, progression to septicemia, and can lead to immunological sequelae, including glomerulonephritis, arthritis and erythema nodosum. Yersinia enterocolitica has been identified in Brazil in fecal material of human patients and animals, the environment in meat and processed pork, as well as in ice used for human consumption; but it is unknown its actual prevalence in refrigerated raw milk. This work aims to detect and identify the presence of Yersinia enterocolitica in 101 samples of bovine milk coming from bulk tanks of properties located in the Midwest region of Sao Paulo State, by inoculating in three different culture media, followed by biochemical and use evidence API 20E identification system, identification of biotypes and serogroups and the conventional Polymerase Chain Reaction (cPCR), both of milk samples from the tank as the suspected isolated colonies; as well as the presence of virulence genes of pathogenic species of Yersinia enterocolitica: inv, ail , YadA, virF, ystA e ystB. It will also be performed antimicrobial susceptibility profile of the isolates and comparison of different diagnostic methods for identification of Y. enterocolitica.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BERTOLINI, AMANDA BEZERRA; ORSI DUTRA, MARCELO AUGUSTO; GUIRALDI, LIVIA MAISA; DOS SANTOS, WESLEY JOSE; MERINO DE MEDEIROS, MARIA IZABEL; LUCHEIS, SIMONE BALDINI. Isolation and identification of Yersinia enterocolitica in bovine milk samples of bulk tanks from dairy farms in Sao Paulo, Brazil. SEMINA-CIENCIAS AGRARIAS, v. 40, n. 2, p. 723-730, . (16/03582-9)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
BERTOLINI, Amanda Bezerra. Isolamento, identificação molecular e detecção de genes de virulência de yersinia enterocolitica em amostras de leite bovino de tanques de expansão de propriedades do Centrooeste Paulista. 2018. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu Botucatu.

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