Evaluation of bone repair in the bone/implant interface in estrogen deficient famale rats untreated and treated with strontium ranelate - analysis three dimensional microtomography and immunohistochemistry

Abstract

The purpose of this study is to evaluate the peri implant bone repair in rats submitted to ovariectomy and treated with Strontium ranelate (625mg / kg / day). Thirty-six (36) female rats, weighing approximately 300 grams, will be divided into 3 groups according to drug treatment: SHAM group, 12 rats submitted to sham surgery; OVX group, 12 rats submitted to ovariectomy surgery and without drug treatment; Group RAN-Sr, 12 rats submitted to ovariectomy surgery and treated with Strontium ranelate. Each animal will receive 2 implants with 1 in each tibial metaphysis. The analyzes to be performed are the microtomography three-dimensional (Micro-CT) and immunohistochemistry for immunoblotting of proteins that characterize the osteoblast differentiation process (will be evaluated RUNX-2, osteopontin and osteocalcin) and alkaline phosphatase. The quantitative data will be subjected to statistical test of normality and homoscedasticity, applied a parametric or non-parametric test. For all data is considered to be 5% significance level.