Evaluation of the transformation potential of toxic compounds by isolates Gordonia paraffinivorans and Gordonia sihwensis deriving from composting

Abstract

Bioremediation is an efficient methodology for recovering polluted environments. It acts employing microorganisms able to degrade toxic compounds transforming them into other substances. Among the microorganisms used in bioremediation, the genus Gordonia has excelled due to its great versatility, allowing metabolic utilization of complex toxic substrates as energy source, removing them from the environment. Sequencing and analysis of the genomes of two isolates of G. paraffinivorans (MTZ 041 and 052) and an isolated of G. sihwensis (MTZ 096) from composting, demonstrated that these microorganisms possess the genetic factors that confer the ability to degrade hydrocarbons, synthetic and natural rubber latex. This master's work proposes to express the alkane hydroxylase system (AH) of G. sihwesis MTZ096 in E. coli, which is known for giving the ability of hydrocarbon's assimilation to other Actinomycetes. In addition, part of the goals of this study are to explore the biodegradable potential of natural rubber and synthetic latex from isolates of G. paraffinivorans. (AU)