Advanced search
Start date
Betweenand

Development of rapid microbiological methods (RMMs) for evaluation of potency of antimicrobial agents and their measurement uncertainties

Grant number: 16/04100-8
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): July 01, 2016
Effective date (End): July 31, 2019
Field of knowledge:Health Sciences - Pharmacy - Medicines Analysis and Control
Principal researcher:Felipe Rebello Lourenço
Grantee:Alessandro Morais Saviano
Home Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Agar diffusion method has been used in the evaluation of antimicrobial activity since the discovery of penicillin. Nevertheless, little progress has occurred in order to reduce the time required for the determination of growth inhibition zones. The goal of this project is to develop, optimize and validate rapid microbiological methods for evaluation of potency of antimicrobials, as well as to identify, quantify and assess the main sources ofuncertainty associated with potency. The project will be divided into four steps: step 1) determination of the parameters involved in the formation of inhibition zones and study of mechanisms of diffusion and microbial growth~ 2) influence of culture medium composition on inhibition zones~ 3) development, optimization and validation of rapid microbiological methods for the determination of antimicrobials potencies~ and 4) study of measurement uncertainty associated with antimicrobials potencies. We hope that the results of this project will allow the reduction of the time required for the determination of inhibition zone sizes. Additionally, we hope to contribute to the elucidation of the mechanisms of diffusion and microbial growth, making it possible to identify,quantify and improve the main sources of measurement uncertainty associated with formation of inhibition. (AU)

News published in Agência FAPESP Newsletter about the scholarship:
Articles published in other media outlets (0 total):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SAVIANO, ALESSANDRO MORAIS; LOURENCO, FELIPE REBELLO. Using image analysis to determine gentamicin potency by agar diffusion microbiological assay and its measurement uncertainty. MEASUREMENT, v. 146, p. 315-321, NOV 2019. Web of Science Citations: 0.
SAVIANO, ALESSANDRO MORAIS; BETTENCOURT DA SILVA, RICARDO J. N.; LOURENCO, FELIPE REBELLO. Measurement uncertainty for the potency estimation by rapid microbiological methods (RMMs) with correlated data. REGULATORY TOXICOLOGY AND PHARMACOLOGY, v. 102, p. 117-124, MAR 2019. Web of Science Citations: 0.
SAVIANO, ALESSANDRO MORAIS; LOURENCO, FELIPE REBELLO. Rapid microbiological methods (RMMs) for evaluating the activity of cephalosporin antibiotics employing triphenyltetrazolium chloride. Talanta, v. 185, p. 520-527, AUG 1 2018. Web of Science Citations: 4.
SAVIANO, ALESSANDRO MORAIS; LOURENCO, FELIPE REBELLO. Measurement uncertainty estimation based on multiple regression analysis (MRA) and Monte Carlo (MC) simulations - Application to agar diffusion method. MEASUREMENT, v. 115, p. 269-278, FEB 2018. Web of Science Citations: 8.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SAVIANO, Alessandro Morais. Development of rapid microbiological methods (RMMs) for evaluation of potency of antimicrobial agents and their measurement uncertainties. 2020. Doctoral Thesis - Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) São Paulo.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.