Brazil is one of the largest producers and exporters of oranges in the world, with the State of São Paulo the largest citrus producer in the country. However, in recent years the citrus genus plants have been strongly affected by various diseases and among these, the HLB disease (HLB) have been highlighted by severely compromising the development of plants, causing losses in fruit production and economic losses to the citrus industry world. The HLB disease, the bacteria Candidatus Liberibacter spp., Colonization and clogs the elaborate sap conducting vessels (phloem) and is transmitted by the vector, the psyllid Diaphorina citri. As the current control strategies have not fully effective against the disease, as well as achieving environmental risks and are costly, development strategies, effective and less harmful to the environment is needed to reduce the economic losses of the world's citrus industry this way, an insect detailed study Diaphorina citri to knowledge of its biology and characteristics is of great interest because it may contribute to the development of tools used to control the HLB (HLB). In a recent study, in collaboration with Professor Andrea Soares Costa of the UFSCar we identify, express and characterized a cathepsin B Diaphorina citri that was called DCCathB. In this context, in this project we will continue the DCCathB studies, seeing a possible target for control of D. citri. In addition, we aim to identify and characterize other cysteine peptidases type cathepsin B and L D. citri, as well as gene expression analysis of these enzymes by RT-qPCR in different stages of development and insect tissues.
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