Extract obtaining and synthesis of silver nanoparticles from Punica granatum (pomegranate): Physico-chemical characterization, determination of antimicrobial activity and potential healing

Abstract

The aim of this study is to produce extracts from the bark of Punica granatum (pomegranate) and synthesize silver nanoparticles from these extracts (synthesis green), characterize them, evaluate the minimum inhibitory concentration aigainst Candida albicans SC5314 and Staphylococcus aureus ATCC 25923 and its effect on wound repair reproduced in mice and infected with the same microorganism. The extracts will be obtained by alcoholic route and will be characterized physico-chemically as pharmacopoeic analysis and high-performance liquid chromatography (HPLC). They will be used to reduce silver nitrate in silver nanoparticles, and the presence of silver nanoparticles will be confirmed by TEM, SEM and UV-Vis spectroscopy. The minimum inhibitory concentration will be determined for all compounds (extracts silver nanoparticles). After the identification of ideal concentrations of the compound a semisolid formulation will be developed and tested in would reproduced in mice verifying its anti-inflammatory potential and also its antimicrobial efficacy against mixed biofilm of C. albicans and S. aureus by counting colony forming units (UFC) in model in vivo. Data will be subjected to normality test and then appropriate statistical analysis will be applied for each outcome with the 5% significance level.