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Assessment of synthetic peptides on directed Hepatitis C virus inhibition

Grant number: 16/02174-4
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): July 01, 2016
Effective date (End): July 31, 2018
Field of knowledge:Biological Sciences - Microbiology
Principal Investigator:Paula Rahal
Grantee:Mariana Nogueira Batista
Host Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil


Hepatitis C is the liver inflammation arising from hepatitis C virus (HCV) infection, frequently it evolves to chronic conditions and has been considered worldwide the major cause of cirrhosis and hepatocellular carcinoma, reported as the main world public health problem. It is estimated that 150 million of people have chronic infection by this vírus and that 3 to 4 million of new cases arise each year. The main HCV transmission pathway is parenteral and current treatments are based on PEG-IFN and ribavirin along with second generation direct acting antivirals. The last ones presents much side-effects, high costs and resistant mutants has been reported. Therefore, new effective treatments, less expensive, with higher spectrum and lower side-effects have been sought. Thus, the aim of this study is to develop a prodrug with directed action for HCV infected cells and efficient against more than one step of viral replication cycle For that, an initial screening will be performed for five synthetic peptides subsequently modeled for prodrugs production, what will be analized using the viral replication models J6/JFH-1 RLUC e JFH-1, the subgenomic replicons SGR-JFH1-Feo (genotype 2) e S52/SG (genotype 3); and the cell line Huh-7.5. The analysis will include citoxicity assay, luciferase-based assay and western blotting for inhibitory capacity of compound on viral replication; indirect immunofluorescence for entry assays and qPCR for compounds effect on viral release step assessment.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BATISTA, MARIANA NOGUEIRA; DA SILVA SANCHES, PAULO RICARDO; CARNEIRO, BRUNO MOREIRA; SILVA BRAGA, ANA CLAUDIA; FERNANDES CAMPOS, GUILHERME RODRIGUES; CHILLI, EDUARDO MAFFUD; RAHAL, PAULA. GA-Hecate antiviral properties on HCV whole cycle represent a new antiviral class and open the door for the development of broad spectrum antivirals. SCIENTIFIC REPORTS, v. 8, . (17/00287-9, 16/02174-4, 13/07600-3, 15/23244-8)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
BATISTA, Mariana Nogueira. Evaluation of synthetic peptides on hepatitis C virus direct inhibition. 2018. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências Letras e Ciências Exatas. São José do Rio Preto São José do Rio Preto.

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Filed patent(s) as a result of this research project

COMPOSTO COM PROPRIEDADES ANTIVIRAIS CONTRA VÍRUS ZIKA BR102021014737-7 - Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp) . Bruno Moreira Carneiro ; Cintia Bittar Oliva ; Eduardo Maffud Cilli ; Gabriela Miranda Ayusso ; Maria Leticia Duarte Lima ; Mariana Nogueira Batista ; Marilia de Freitas Calmon ; Paula Rahal ; Paulo Ricardo da Silva Sanches - July 2021, 27