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Expression, purification and kinetics and immunogenic characterization of CysA2, a putative rhodanese-like protein involved in the sulfur metabolism from Mycobacterium tuberculosis

Grant number: 16/03196-1
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): June 01, 2016
Effective date (End): May 31, 2017
Field of knowledge:Biological Sciences - Biochemistry - Chemistry of Macromolecules
Principal Investigator:Andrea Balan Fernandes
Grantee:Caio Cesar Nogueira Cambui
Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil


Tuberculosis is an infection caused by the bacillus Mycobacterium tuberculosis. This disease is one of the greatest problems faced by the world, according to the World Health Organization. Due to its complex pharmacological regimen and the high incidence of adverse reactions, the compliance of patients to the treatment is often low, fact that facilitates and promotes the selection of resistant and multi-resistant strains of this bacterium. Sulfur and the sulfur pathways are essential for the survival of M. tuberculosis. CysA2 is a putative thiosulfate sulfurtransferase rhodanese-like (rhodanase) enzyme that seems to be involved in the sulfur metabolism. Because of the key role of sulfur metabolism in the bacterium and the lack of a similar pathway in humans, the development of drugs able to target components of this pathway represents an important step towards the elaboration of new ways to combat this disease. Our group works with both functional and structural characterization of enzymes involved with the sulfur metabolism. CyA2 was produced was produced from Escherichia coli in a stable and soluble form. Since there are data about its structure, but not about its functions, our group aims to characterize the kinetics and the pro-inflammatory activity of this enzyme and, hopefully, the knowledge that will be generated will contribute to the future development of new and more effective ways of treating tuberculosis.

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