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Cloning, expression, purification, biophysical and functional characterization of members of the human Hsp110 family co-chaperone with emphasis on its role in protein disaggregation systems

Grant number: 16/04246-2
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): June 01, 2016
Effective date (End): June 11, 2017
Field of knowledge:Biological Sciences - Biochemistry - Chemistry of Macromolecules
Principal Investigator:Carlos Henrique Inacio Ramos
Grantee:Gabriela de Mello Gandelini
Host Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Incorrect protein folding can lead to many diseases and even aging. Loss of protein function and formation of amyloidogenic protein aggregates are also consequences of protein misfolding. In order to protect these aggregates, many organisms express members of the Hsp100 molecular chaperones family (ClpB in bacteria, Hsp104 in yeast and Hsp101 in plants, for example), which are able to disaggregate other proteins, leading to their reactivation. Surprisingly, metazoan do not have a gene for Hsp100. However, recent studies have shown that a family member of the Hsp110 co-chaperone, along with Hsp70 and Hsp40 chaperones, cooperate in a system with desagregase function. Our research group has already expressed and characterized the human Hsp70 and Hsp40 chaperones, both in structural and functional terms. Recently, we have obtained three cDNAs from the human Hsp110 family members, and only one of these members was tested for desagregase activity. Thus, this project has the aim of investigate the chaperone activity of the other two members not studied yet. Hence, we will clone, express, purify, characterize and test them, both alone and with other chaperones, about the desagregase function. The results have the potential to increase significantly the knowledge about protein homeostasis in the cell, which can generate inputs that have medical and biotechnological application. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FRANCO, JULIANA C.; NOGUEIRA, MARIA L. C.; GANDELINI, GABRIELA M.; PINHEIRO, GLAUCIA M. S.; GONCALVES, CONRADO C.; BARBOSA, LEANDRO R. S.; YOUNG, JASON C.; RAMOS, CARLOS H. I.. Sorghum bicolor SbHSP110 has an elongated shape and is able of protecting against aggregation and replacing human HSPH1/HSP110 in refolding and disaggregation assays. Biopolymers, v. 114, n. 2, p. 12-pg., . (16/02137-1, 12/50161-8, 16/14503-2, 16/04246-2, 16/03764-0, 18/11948-9, 17/26131-5)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
GANDELINI, Gabriela de Mello. Expression, purification, conformational and functional characterization of HSPH1, a human HSP110 co-chaperone, with emphasis on its role in protein disaggregation systems. 2018. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Instituto de Química Campinas, SP.

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