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Influence of seminal plasma from rich fraction of boar ejaculated on capacitation and hyperactivation of the swine sperm stored at 17ºC for 72 hours

Grant number: 16/02186-2
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): May 01, 2016
Effective date (End): August 31, 2018
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Reproduction
Principal Investigator:André Furugen Cesar de Andrade
Grantee:Ana Paula Pinoti Pavaneli
Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated scholarship(s):17/20419-7 - Influence of seminal plasma during cooling of boar spermatozoa on dna integrity, sperm subpopulations and calcium influx under in vitro capacitation., BE.EP.MS


The present project aims to evaluate the effect of seminal plasma from rich fraction of boar ejaculated on capacitation and sperm hyperactivation of semen stored at 17ºC for 72 hours. For this, six ejaculates of each of six mature boars will be collected by gloved-hand technique. Semen in natura will be assessed to the motility characteristics by computer-assisted sperm analysis (CASA), sperm morphology by differential interference contrast (DIC) and sperm concentration by Neubauer chamber. The ejaculate will be split into three conical 50 mL tubes, originating three treatments: non-centrifuged (NC), centrifuged followed by removal of seminal plasma after centrifugation (CS), centrifuged with suspended seminal plasma (CR). The treatments CS and CR will be subjected to centrifugation at 500xg for 10 minutes. All the treatments will be extended with Beltsville Thawing Solution (BTS) to produce an insemination dose with 30x106 spermatozoa/ml in 50 ml. Then, semen will be held for 90 minutes in the dark at room temperature before storage at 17º C for 72 hours. After this, the samples will be transferred to capacitating medium followed by incubation at 38º C with 5% CO2 and evaluated in the intervals of 0, 2 and 4 hours for the characteristics: plasma membrane fluidity (capacitation), presence of phosphorylation of tyrosine aminoacid, acrossomal integrity (acrossome reaction), hyperactivation, and plasmatic membrane and cytoplasm peroxidation. When the main effect is significant, the means will be compared by Tukey test. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PINOTI PAVANELI, ANA PAULA; RECUERO, SANDRA; RESENDE CHAVES, BRUNA; GARCIA-BONAVILA, ESTELA; LLAVANERA, MARC; PINART, ELISABETH; ETBON, SERGI; CESAR DE ANDRADE, ANDRE FURUGEN; YESTE, MARC. The Presence of Seminal Plasma during Liquid Storage of Pig Spermatozoa at 17 degrees C Modulates Their Ability to Elicit In Vitro Capacitation and Trigger Acrosomal Exocytosis. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 21, n. 12, . (16/02186-2, 17/20419-7, 15/14258-5)
PINOTI PAVANELI, ANA PAULA; PASSARELLI, MARINA DA SILVA; DE FREITAS, FLAVIA VIEIRA; RAVAGNANI, GISELE MOURO; TORRES, MARIANA ANDRADE; MASSAMI KITAMURA MARTINS, SIMONE MARIA; YESTE, MARC; CESAR DE ANDRADE, ANDRE FURUGEN. Removal of seminal plasma prior to liquid storage of boar spermatozoa: A practice that can improve their fertilizing ability. Theriogenology, v. 125, p. 79-86, . (15/14258-5, 16/02186-2)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
PAVANELI, Ana Paula Pinoti. Influence of seminal plasma from sperm-rich fraction of ejaculate on capacitation and hyperactivation of boar sperm stored at 17 °C for 72 hours. 2018. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) Pirassununga.

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