Advanced search
Start date
Betweenand

Effects of ascorbic acid in the myogenesis of skeletal muscle of pacu (Piaractus mesopotamicus)

Grant number: 16/01086-4
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): April 01, 2016
Effective date (End): March 31, 2017
Field of knowledge:Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
Principal researcher:Maeli Dal Pai
Grantee:Guilherme Alcarás de Góes
Home Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The pacu (Piaractus mesopotamicus) is a fish species which has several economically interesting features for aquaculture, with high commercial value and object of an intense research to improve its production. The skeletal muscle is the most abundant tissue in teleost fish, representing up to 65% of the total body mass for some species. Fast muscle comprises the bulk of the fillet and corresponds to the commercial part of the fish. The skeletal muscle growth involves the myoblasts, which through proliferation, migration and differentiation promote the muscle hyperplasia and hypetrophy. More and more studies use the myoblast cell culture from fish as a tool to evaluate the muscle growth regulation, because it recapitulates the steps of myogenesis. Besides, the myoblast cell culture can be manipulated to evaluate the role of nutrients and growth factors in a controlled environment. The muscle growth is a multifactorial process regulated by transcriptional factors, hormones, cytokines and nutrients. Among them we highlight the ascorbic acid an essential vitamin in fish diet and whose lack leads to diseases, anatomical deformity and impairment of the development. The ascorbic acid acts as an antioxidant and also in collagen synthesis to structure and maintenance of connective tissue and osteogenesis. However, it is unclear whether ascorbic acid can directly influence myogenesis of skeletal muscle of fish and, consequently, contribute to muscle growth of the animal. Our hypothesis is that ascorbic acid contributes to increase myoblast proliferation and migration during myogenesis and muscle growth pacu. In this work we intend to evaluate the role of ascorbic acid on the proliferation and migration of myoblasts from pacus in primary cell cultures. Our study will contribute to a better understanding of the myogenesis regulation in fish, allowing the development of a theoretic framework that may help to improve muscle growth.

News published in Agência FAPESP Newsletter about the scholarship:
Articles published in other media outlets (0 total):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SILVA DURAN, BRUNO OLIVEIRA; GOES, GUILHERME ALCARAS; THOMAZINI ZANELLA, BRUNA TEREZA; FREIRE, PAULA PACCIELLI; VALENTE, JESSICA SILVINO; SIMOES SALOMAO, RONDINELLE ARTUR; FERNANDES, ANA; MARECO, EDSON ASSUNCAO; CARVALHO, ROBSON FRANCISCO; DAL-PAI-SILVA, MAELI. Ascorbic acid stimulates the in vitro myoblast proliferation and migration of pacu (Piaractus mesopotamicus). SCIENTIFIC REPORTS, v. 9, FEB 18 2019. Web of Science Citations: 2.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.