Diagnostic of Brazilian and emergent arboviruses in patients and mosquitoes from two distinct regions in Brazil

Abstract

Arboviruses are frequently associated with outbreaks in humans and represent a serious public health problem, with economic and social impacts. Among the Brazilian arboviruses, Mayaro virus, Dengue virus, Yellow Fever virus, Rocio virus and Oropouche virus are responsible for more than 95% of human cases in Brazil. These arboviruses usually present the same clinical symptoms, especially on the acute phase of infection. Today, Brazil is facing a large Dengue outbreak; however, other arboviruses were diagnosed together like Chikungunya and Zika virus. Amazon region is an important area for arboviruses, with the entire basic element to maintain the viral cycle. Equally, São José do Rio Preto, state of São Paulo, is important to the arboviruses surveillance because it is hyper endemic to Dengue and presents circulation of Saint Louis Encephalitis virus and possible other arboviruses for presenting high infestation of the vectors A. aegypti e Culex sp. The aim of this study is to investigate the presence of arboviruses in mosquitoes and patients presenting acute febrile illness in the cities of São José do Rio Preto (SP) and Manaus (AM), two areas with distinct environmental and epidemiological profile but with the presence of Dengue outbreak. The identification of the main Brazilian and emergent arboviruses associated to the vector data could be compared to the results from cohort study performed in the two cities to describe the entrance and circulation of the viral agents in the country. The data will provide tools to help in the surveillance and prevention of outbreaks. (AU)