Penile carcinoma (PeCa) is a frequent disease in poor and developing countries showing high morbidity. Despite of the progress in the molecular research of PeCa, including very recently published high-throughput studies conducted by our group, the lack of well characterized in vitro models precludes new advances in the knowledge of cellular processes as well as in preclinical tests for anticancer drugs efficacy. During the post-doctoral period, we established and characterized six cell cultures derived from penile carcinomas aiming to provide reliable in vitro models for functional and pre-clinical studies in PeCa. The objective of this BEPE internship is to perform high-throughput methods to molecularly characterize these cell cultures. Since multiple mechanisms can modify gene expression and promote carcinogenesis, a multidimensional integrative analysis will be performed for a more comprehensive understanding of the signaling pathways that contribute to penile tumorigenesis. Transcriptomic and translatomic (HTA 2.0 Affymetrix), genomic (Cytoscan-Affymetrix), epigenomic (Infinium® Human Methylation450 BeadChip) and proteomic (Reverse phase protein array - RPPA) approaches will be performed in the six PeCa cell cultures and two foreskin cell lines. Integration of all omics profiles and interactome analysis will be performed in collaboration with Dr Jan Baumbach, University of Southern Denmark, DK. In addition, the data herein obtained will be compared with results previously obtained by our group in PeCa fresh tissues. From these analyses, we intend to reveal altered pathways in PeCa that could be used to develop targeted treatments. In addition, we also aim to investigate the potential of these cell models to be used in preclinical trials of PeCa.
News published in Agência FAPESP Newsletter about the scholarship: