Concurrent with the population growth, agricultural production tend to grow to meet the food demand. To reset the macro and micronutrients that are removed from the soil in crops, the application of chemical fertilizers are constantly applied. The indiscriminate use of inputs, particularly phosphate, is responsible for the appearance of many impacts on the environment. In order to minimize the effects of this type of fertilizer, the application of microorganisms that promote nutrient absorption is to be tested as a strategy to be tested. It is believed that low phosphorus concentration in soil, enriches the rhizosphere microbiome related to the availability of this element to the plant. Thus, this project aims to evaluate the rhizosphere microbiome contribution in P uptake by plants considering two contrasting cultivars in the uptake efficiency of this element and different concentrations of phosphate rock and super-simple. Bacteria with potential in the phosphorus solubilization will be identified through rhizosphere soil cultivation in specific media with calcium phosphate and phytate. The isolate will be characterized about organic acid production and identified through 16S gene sequencing. Microbial community structure will be accessed through 16S gene sequencing of metagenomic DNA extracted from rhizosphere soil, and the presence of mycorrhizal fungi will be quantified through qPCR. The search for approaches that aim to improve soil fertility, employing the most efficient use of nutrients, may be a promising way to the intensification of an environmentally sustainable agriculture.
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