The aim of this study will be to propose the synthesis and characterization of a nanocomposite based on silver nanoparticles and B-calcium glycerophosphate nanoparticles (Ag/B-Calcium glycerophosphate), evaluate the action of this nanocomposite on Candida albicans and Streptococcus mutans. First, colloidal silver nanoparticles (5 nm) will be synthesized by reduction of the silver nitrate through sodium citrate. B-Calcium glycerophosphate nanoparticles will be synthesized by grinding conventional particles of B-Calcium glycerophosphate. These two compounds will be associated seeking a concentration of 9 wt% of phosphate. During the nanocomposite synthesis X-rays diffraction and transmission electronic microscopy will be performed. Subsequently, the effect of different concentrations and periods of treatments with nanocomposite on the OD-21 cells (1) analysis of cell proliferation by MTT, (2) alkaline phosphatase activity assessment by thymolphthalein release by hydrolysis of thymolphthalein monophosphate, (3) detection and quantification of mineralization by alizarin red staining. Controls devoid B-calcium glycerophsphate and with calcium hydroxide will be included in all analyzes, and all tests will be performed in triplicate on three different occasions. Data will be analyzed to employ the most appropriate statistical test. P< .05 will be considered statistically significant.
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