Scholarship 15/21562-2 - Estresse oxidativo, Poluição ambiental - BV FAPESP
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Synthesis of phytochelatins and expression of genes involved in the control of oxidative stress and in the cadmium detoxification in Panicum maximum Jacq. cv. Tanzania supplied with sulfur

Grant number: 15/21562-2
Support Opportunities:Scholarships abroad - Research Internship - Doctorate
Start date until: July 01, 2016
End date until: February 28, 2017
Field of knowledge:Biological Sciences - Physiology - General Physiology
Principal Investigator:José Lavres Junior
Grantee:Flávio Henrique Silveira Rabêlo
Supervisor: Ann Cuypers
Host Institution: Centro de Energia Nuclear na Agricultura (CENA). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
Institution abroad: Hasselt University, Diepenbeek, Belgium  
Associated to the scholarship:14/16731-7 - Relationship between sulfur and cadmium (111Cd) detoxification mechanisms in Panicum maximum cv. Tanzania, BP.DR

Abstract

The cadmium (Cd) concentration in the environment has increased in the last few years, resulting in serious socio-environmental problems such as lower mass production from plants cultivated in contaminated areas and the entry of these toxic elements into the food chain through the consumption of contaminated foods, which can cause numerous health problems. Thus, the use of plants able to accumulate significant amounts of heavy metals is a very promising alternative to treat contaminated environments, especially if the adequate level of sulfur (S) is provided. Glutathione (GSH) and the phytochelatins (PCs) are metabolites known to work in the detoxification of Cd and are involved in the tolerance of plants to this toxic metal, and thus an increase in the synthesis of these metabolites by plants under stressful conditions is desirable. Therefore, the adequate supply of S is essential, given that this element is necessary for the synthesis of cysteine, a structural amino acid of the thiol group of GSH and PCs. However, when the Cd concentration in the cellular environment is too high and the detoxification of this metal by GSH and PCs is insufficient, oxidative damage may occur resulting from the imbalance between pro-oxidants and antioxidants. Cadmium can also change the expression of specific genes, which are necessary for the synthesis of GSH, of PCs, and of antioxidant enzymes. Our objective with this study hence will be to quantify the synthesis of phytochelatins PC2, PC3, and PC4 and the expression of genes GSH1, GSH2, PCS1, PCS2, CSD1, CSD2, CSD3, FSD1, FSD2, FSD3, MSD1, APX1, GR1, GR2, CAT1, CAT2, and CAT3, aiming to understand how the adequate supply of S can interfere with the detoxification of Cd and with the combat against reactive oxygen species (ROS) in Panicum maximum Jacq. cv. Tanzania plants exposed to Cd. This study will be developed in pots cultivated in a greenhouse, where a nutrient solutions will be supplied containing three levels of S (0.1, 1.9, and 3.7 mmol L-1) and three levels of Cd (0.0, 0.1, and 0.5 mmol L-1). After the transplanting of five plantlets into each pot, solutions diluted at 25% ionic strength and modified to meet the S dose will be applied. Undiluted nutrient solutions modified to meet the S doses will be supplied from the eight day, for 14 days. From the 21st day, the undiluted nutrient solutions modified to meet the S and Cd doses will be supplied until harvest. During the harvest, the plant material will be separated into leaf, stem, and root, frozen in liquid N, and subsequently sent for analyses of PCs and gene expression. Results will be subjected to analysis of variance, and treatment means will be compared by Tukey's test at 5% significance level using SAS statistical software, in which studies of correlation between the analyzed variables will also be carried out. (AU)

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Scientific publications (7)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SILVEIRA RABELO, FLAVIO HENRIQUE; BORGO, LUCELIA; KEUNEN, ELS; DOS REISE, ANDRE RODRIGUES; MARTINELLI, ADRIANA PINHEIRO; CUYPERS, ANN; LAVRES, JOSE. Enzymatic antioxidants-Relevant or not to protect the photosynthetic system against cadmium-induced stress in Massai grass supplied with sulfur?. ENVIRONMENTAL AND EXPERIMENTAL BOTANY, v. 155, p. 702-717, . (14/18735-0, 17/12367-7, 14/16731-7, 15/21562-2)
RABELO, FLAVIO HENRIQUE SILVEIRA; VANGRONSVELD, JACO; BAKER, ALAN J. M.; VAN DER ENT, ANTONY; ALLEONI, LUIS REYNALDO FERRACCIU. Are Grasses Really Useful for the Phytoremediation of Potentially Toxic Trace Elements? A Review. FRONTIERS IN PLANT SCIENCE, v. 12, . (14/18735-0, 12/11859-0, 15/21562-2, 18/07190-3, 17/11299-8, 12/12894-3, 14/16731-7)
RABELO, FLAVIO HENRIQUE SILVEIRA; LAVRES, JOSE; RAMIRES, THIAGO GENTIL; ALLEONI, LUIS REYNALDO FERRACCIU. Diagnosis and recommendation integrated system and nutritional balance index reveal Cd-induced nutritional disorders in Panicum maximum assayed for Cd phytoextraction. BIOREMEDIATION JOURNAL, v. 24, n. 4, p. 265-282, . (14/18735-0, 15/21562-2, 12/12894-3, 17/11299-8, 14/16731-7, 12/11859-0, 18/07190-3)
SILVEIRA RABELO, FLAVIO HENRIQUE; MORAL, RAFAEL DE ANDRADE; LAVRES, JOSE. Integrating Biochemical, Morpho-physiological, Nutritional, and Productive Responses to Cd Accumulation in Massai Grass Employed in Phytoremediation. WATER AIR AND SOIL POLLUTION, v. 230, n. 5, . (15/21562-2, 14/18735-0, 14/16731-7, 14/03310-3)
SILVEIRA RABELO, FLAVIO HENRIQUE; JORDAO, LUIZ TADEU; LAVRES, JOSE. A glimpse into the symplastic and apoplastic Cd uptake by Massai grass modulated by sulfur nutrition: Plants well-nourished with S as a strategy for phytoextraction. Plant Physiology and Biochemistry, v. 121, p. 48-57, . (15/21562-2, 14/18735-0, 14/16731-7)
SILVEIRA RABELO, FLAVIO HENRIQUE; LUX, ALEXANDER; ROSSI, MONICA LANZONI; MARTINELL, ADRIANA PINHEIRO; CUYPERS, ANN; LAVRES, JOSE. Adequate S supply reduces the damage of high Cd exposure in roots and increases N, S and Mn uptake by Massai grass grown in hydroponics. ENVIRONMENTAL AND EXPERIMENTAL BOTANY, v. 148, p. 35-46, . (15/21562-2, 14/18735-0, 14/16731-7)
SILVEIRA RABELO, FLAVIO HENRIQUE; FERNIE, ALISDAIR ROBERT; NAVAZAS, ALEJANDRO; BORGO, LUCELIA; KEUNEN, ELS; DE ALCANTARA DA SILVA, BERENICE KUSSUMOTO; CUYPERS, ANN; LAVRES, JOSE. A glimpse into the effect of sulfur supply on metabolite profiling, glutathione and phytochelatins in Panicum maximum cv. Massai exposed to cadmium. ENVIRONMENTAL AND EXPERIMENTAL BOTANY, v. 151, p. 76-88, . (15/21562-2, 14/18735-0, 14/16731-7)

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