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Evaluation of bone quality of human dental pulp stem cells seeded on biomaterials with potential application in bone bioengineering

Grant number: 15/18661-9
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): December 01, 2015
Effective date (End): February 28, 2017
Field of knowledge:Health Sciences - Dentistry
Principal researcher:Katiúcia Batista da Silva Paiva
Grantee:Letícia Marinho Silva Rocha
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:10/08918-9 - Gene-modified-tissue engineering of bone: overexpression and inhibition of matrix metalloproteinases (MMPs) and their inhibitors (TIMPs and RECK) by osteoblasts from human mesenchymal stem cells seeded on collagen-chitosan, AP.JP


Bone quality is determined by composition and bone structure that contributes to strength regardless of bone mineral density, which include: bone turnover (balance between bone formation and resorption) microarchitecture, mineralization, microdamages and composition of the organic and inorganic bone extracellular matrix (ECM). Tissue Bioengineering aims to develop new therapies that replace, restore or improve tissue function using the individual's own cells associated with suitable biomaterials and stimulating them with bioactive molecules to produce new tissue in vitro. In the Bioengineering bone context, bone quality is an important parameter to guide the development of in vitro systems capable of mimicking more and more bone tissue in vivo. Our group has evaluated the influence of MMPs and their inhibitors in modifying bone ECM composition for the induction of osteoblast differentiation in vitro from human dental pulp stem cells (DPSCs) as well as when seeded on the biomaterial collagen-chitosan. Our data indicate that they are differentially expressed during differentiation and modulating the ECM, thus leading to a possible change in bone architecture. This project aims to determine whether the modulation of the expression of MMPs, TIMPs and RECK, either by overexpression or gene silencing, during osteogenesis in vitro and in vivo from the seeded DPSCs on the blend of collagen type I / chitosan can change the parameters of bone quality.

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