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Polymeric microparticles as the fungus Trichoderma harzianum carrier system aimed at applications in agriculture

Grant number: 15/18231-4
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): January 01, 2016
Effective date (End): June 30, 2019
Field of knowledge:Biological Sciences - Biochemistry - Chemistry of Macromolecules
Principal Investigator:Leonardo Fernandes Fraceto
Grantee:Cintia Rodrigues Maruyama
Host Institution: Instituto de Ciência e Tecnologia. Universidade Estadual Paulista (UNESP). Campus de Sorocaba. Sorocaba , SP, Brazil


Demand from the use of products for sustainable agriculture and causing less impact on the environment is increasingly larger. Thus, the use of biological control is an alternative to reduce the use of pesticides and the risks they entail. The fungus Trichoderma harzianum is an example of effective biological control against the pathogen Sclerotinia sclerotiorum, which affects many crops and brings production losses of up to 100%. However, the use of this biocontrol fungus may encounter some problems, for example, fungal stress biotic and abiotic factors, degração by UV light, temperature and lack of moisture. One solution to this problem would be the microenpcaulação biological control of fungus in microparticles. Thus, this work aims to develop microparticles of calcium alginate as a carrier of fungal system, characterize the micro system through physicochemical methods, analyze the biological activity of Trichoderma harzianum fungus free and microenpcasulado on the fungus Sclerotinia sclerotiorum order to obtain information on the carrier system, in addition to soil microbiota assessment to verify the effects of carrier systems on the bacterial community, for the development of safer and effective alternatives to control the pathogen fungus, thus reducing environmental impacts.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FRACETO, LEONARDO F.; MARUYAMA, CINTIA R.; GUILGER, MARIANA; MISHRA, SANDHYA; KESWANI, CHETAN; SINGH, HARIKESH BAHADUR; DE LIMA, RENATA. Trichoderma harzianum-based novel formulations: potential applications for management of Next-Gen agricultural challenges. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, v. 93, n. 8, p. 2056-2063, . (16/13692-6, 15/15617-9, 15/18231-4)
MARUYAMA, CINTIA RODRIGUES; BILESKY-JOSE, NATALIA; DE LIMA, RENATA; FRACETO, LEONARDO FERNANDES. Encapsulation of Trichoderma harzianum Preserves Enzymatic Activity and Enhances the Potential for Biological Control. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, v. 8, . (17/21004-5, 15/18231-4)
GERMANO-COSTA, T.; BILESKY-JOSE, N.; GUILGER-CASAGRANDE, M.; PASQUOTO-STIGLIANI, T.; ROGERIO, CB.; ABRANTES, DC.; MARUYAMA, CR.; OLIVEIRA, JL.; FRACETO, LF.; LIMA, R.. Use of 2D and co-culture cell models to assess the toxicity of zein nanoparticles loading insect repellents icaridin and geraniol. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 216, p. 12-pg., . (20/05816-2, 17/20932-6, 18/14734-0, 15/18231-4, 17/25702-9, 18/02404-5)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
MARUYAMA, Cintia Rodrigues. Polymeric microparticles as a carrier system of the fungus Trichoderma harzianum for agricultural applications.. 2019. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Ciência e Tecnologia. Sorocaba Sorocaba.

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