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Preparation and biological evaluation of alkyl dihydroxybenzoates (AD) as useful agents to combat citrus canker

Grant number: 15/08089-6
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): December 01, 2015
Effective date (End): July 31, 2019
Field of knowledge:Agronomical Sciences - Agronomy - Plant Health
Principal researcher:Luis Octávio Regasini
Grantee:Ana Carolina Nazaré
Home Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil

Abstract

Citrus canker is one of the most aggressive among citrus disease, affecting all types of important citrus crops. The disease, caused by bacillus Xanthomonas citri subsp. citri (Xac), occurs in the central orange juice producing countries, including Brazil and USA. For the canker chemical control, inefficient substances have been used, including copper-containing compounds. Thus, efforts are urgently necessary to identify and develop innovative anti-Xac compounds. First, we will prepared 36 alkyl dihydroxybenzoates (AD), which will be evaluated against Xac by in vitro assays. The AD toxicity will be evaluated against human hepatocarcinoma cells (HepG2). The selectivity index (SI) of AD will be calculated from the values of concentration able to kill 50% of hepatocytes (CC50) and values of minimum bactericidal concentration (MBC). SI = CC50/MBC. Compounds with values of SI equal or superior 10 will be submitted to inhibitory bacterial division assays, using FtsZ sedimentation and GTP hydrolysis methods. After in vitro assays, the three top compounds with highest SI values will be submitted to in vivo assays, including anti-citrus canker on Citrus limonia leaves, phytotoxicity against cucumber (Cucumis sativus) and corn (Zea mays), as well as toxicity against zebrafish (Danio rerio). The data obtained from previous research of our group, involving in vitro and in vivo activity demonstrated by compounds strucuturally similar to AD, and the data obtained by postgraduate student, as well as the insert of this investigation into Fapesp-NWO project corroborate the praticability and internationalization of our proposal. The conclusion of this project led to results of scientific relevance and interest by research, development and innovation sections of industry of pesticides.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
POLAQUINI, CARLOS R.; MORAO, LUANA G.; NAZARE, ANA C.; TORREZAN, GUILHERME S.; DILARRI, GUILHERME; CAVALCA, LUCIA B.; CAMPOS, DEBORA L.; SILVA, ISABEL C.; PEREIRA, JESSE A.; SCHEFFERS, DIRK-JAN; DUQUE, CRISTIANE; PAVAN, FERNANDO R.; FERREIRA, HENRIQUE; REGASINI, LUIS O. Antibacterial activity of 3,3 `-dihydroxycurcumin (DHC) is associated with membrane perturbation. BIOORGANIC CHEMISTRY, v. 90, n. SI SEP 2019. Web of Science Citations: 0.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
NAZARÉ, Ana Carolina. Preparation and evaluation of alkyl dihydroxybenzoates against xanthomonas citri subsp. citri and control of citrus canker. 2019. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências Letras e Ciências Exatas. São José do Rio Preto São José do Rio Preto.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.