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Electrospinning of novel functional nanobiomaterials based on peptides and bottlebrush polymers for tissue engineering

Grant number: 15/09697-0
Support Opportunities:Scholarships abroad - Research
Effective date (Start): July 01, 2016
Effective date (End): June 30, 2017
Field of knowledge:Engineering - Materials and Metallurgical Engineering - Nonmetallic Materials
Principal Investigator:Anderson de Oliveira Lobo
Grantee:Anderson de Oliveira Lobo
Host Investigator: Ali Khademhosseini
Host Institution: Instituto de Pesquisa e Desenvolvimento (IP&D). Universidade do Vale do Paraíba (UNIVAP). São José dos Campos , SP, Brazil
Research place: Harvard University, Cambridge, United States  
Associated research grant:11/17877-7 - Development of new polymeric scaffolds by electrospinning technique with incorporation of vertically aligned carbon nanotubes and nanohidroxyapatite for bone tissue regeneration, AP.JP


This BPE application is directly associated to two other approved grant coordinated by proponent, follows: (i) FAPESP-MIT "Novel Nanobiomaterials Based on Tridimensional Peptides with Drug Delivery Properties to Tissue Engineering" (2014/50720-2) proposal and (ii) Young Researcher FAPESP project "Development of Novel Polymeric Scaffolds by Electrospinning with Incorporated Vertically-Aligned Carbon Nanotubes and Nanohidroxyapatite for Bone Regeneration" (2011/17877-7). In particular, herein we propose to extend this cooperation with the inclusion of new polymers for the controlled release of therapeutic peptides and drugs. This new direction will significantly expand the utility of original work, and open it to new medical applications. Initially, the submitted proposal was based on polymer scaffold fabrication with the incorporation of nanoparticles by electrospinning technique for bone tissue regeneration. The principal investigator used superhydrophilic vertical aligned carbon nanotubes, dispersed carbon nanotubes, TiO2 and nanohydroxyapatite nanoparticles. We obtained robust nanofibers using electrospinning and rotary-jet spinning techniques. Also, we produced nanofibers with incorporated nanoparticles up to 5% (w/w). This result was very attractive to obtained superhydrophilicity, cellular adhesion, calcification of human osteoblasts and bone regeneration. However, we used synthetic and commercial polymers, which precluded the development of new nano-biomaterials relevant to tissue engineering. The Johnson group at MIT has developed new polymeric nano-architectures with applications suitable for peptide and drug delivery. Thus, in this proposal, we seek to leverage the expertise of both groups to develop next-generation fibers using our established electrospinning techniques and Johnson's polymeric nanoparticles. Also will be proposed collaboration with Dr. Khademhosseini at Harvard University due to your large experience and facilities to produce nanobiomaterials using bioprinting process and to in vitro studies of these new produce nanobiomaterials. The proposed research stage scholarship will be very interesting to involved research's groups, because the Laboratory of Biomedical Nanotechnology coordinated by this proponent have experience in polymer extrusion using electrospinning and rotary-jet spinning, which will augment Johnson's current research program. Also, the undergraduate and Ph.D students will perform biological in vitro and in vivo assays, which will lead to rapid translation of our novel materials. This project presents an innovative technology for manufacturing nanofiber scaffolds. We expect to develop a new three-dimensional nanobiomaterial with biomimetic properties that improve bone tissue regeneration and reduce the inflammation process. Thus, following the methodology of this project it is expected that this new nanomaterial can: accelerate the regeneration of bone tissue, recruit stem cells, reduce the risk of bacterial contamination and be sufficiently low-cost to be translated into clinical regenerative medicine applications. In addition, will be expected with the establishment this proposal will can help the proponent to create a new research line at University of Vale do Paraiba, seeking the production of new biomimetic materials based on three -dimensional peptide nanobiomaterials. Finally, we will intend to engage undergraduate students, master's and doctoral degree from Materials Science and biomedical engineering programs. We seek above all to increase the internationalization of graduate and undergraduate programs involving nanobiomaterials applied to tissue engineering researches at University of Vale do Paraiba. (AU)

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Scientific publications (16)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
RODRIGUES, BRUNO V. M.; RAZZINO, CLAUDIA A.; OLIVEIRA, FRANCILIO DE CARVALHO; MARCIANO, FERNANDA R.; LOBO, ANDERSON O.. On the design and properties of scaffolds based on vertically aligned carbon nanotubes transferred onto electrospun poly (lactic acid) fibers. MATERIALS & DESIGN, v. 127, p. 10-pg., . (15/09697-0, 16/00575-1, 15/08523-8, 11/17877-7)
DIAS, VANESSA; MACIEL, HOMERO; FRAGA, MARIANA; LOBO, ANDERSON O.; PESSOA, RODRIGO; MARCIANO, FERNANDA R.. Atomic Layer Deposited TiO2 and Al2O3 Thin Films as Coatings for Aluminum Food Packaging Application. MATERIALS, v. 12, n. 4, . (14/18139-8, 11/50773-0, 11/17877-7, 12/15857-1, 11/20345-7, 16/00575-1, 15/05956-0, 15/09697-0, 18/01265-1, 15/10876-6)
LOPES, F. S.; OLIVEIRA, J. R.; MILANI, J.; OLIVEIRA, L. D.; MACHADO, J. P. B.; TRAVA-AIROLDI, V. J.; LOBO, A. O.; MARCIANO, F. R.. Biomineralized diamond-like carbon films with incorporated titanium dioxide nanoparticles improved bioactivity properties and reduced biofilm formation. Materials Science & Engineering C-Materials for Biological Applications, v. 81, p. 373-379, . (12/15857-1, 11/17877-7, 11/20345-7, 14/11491-8, 15/09697-0, 16/00575-1)
SILVA, EDMUNDO; REIS DE VASCONCELLOS, LUANA MAROTTA; RODRIGUES, BRUNO V. M.; DOS SANTOS, DANILO MARTINS; CAMPANA-FILHO, SERGIO P.; MARCIANO, FERNANDA ROBERTA; WEBSTER, THOMAS J.; LOBO, ANDERSON OLIVEIRA. PDLLA honeycomb-like scaffolds with a high loading of superhydrophilic graphene/multi-walled carbon nanotubes promote osteoblast in vitro functions and guided in vivo bone regeneration. Materials Science & Engineering C-Materials for Biological Applications, v. 73, p. 31-39, . (11/17877-7, 11/20345-7, 15/09697-0, 15/08523-8, 16/00575-1)
RODRIGUES, BRUNO V. M.; RAZZINO, CLAUDIA A.; OLIVEIRA, FRANCILIO DE CARVALHO; MARCIANO, FERNANDA R.; LOBO, ANDERSON O.. On the design and properties of scaffolds based on vertically aligned carbon nanotubes transferred onto electrospun poly (lactic acid) fibers. MATERIALS & DESIGN, v. 127, p. 183-192, . (15/08523-8, 11/17877-7, 15/09697-0, 16/00575-1)
RICCI, R.; LEITE, N. C. S.; DA-SILVA, N. S.; PACHECO-SOARES, C.; CANEVARI, R. A.; MARCIANO, F. R.; WEBSTER, T. J.; LOBO, A. O.. Graphene oxide nanoribbons as nanomaterial for bone regeneration: Effects on cytotoxicity, gene expression and bactericidal effect. Materials Science & Engineering C-Materials for Biological Applications, v. 78, p. 341-348, . (11/17877-7, 11/20345-7, 13/14464-9, 13/20054-8, 15/09697-0, 09/18440-1, 16/00575-1)
SANTANA-MELO, GABRIELA F.; RODRIGUES, BRUNO V. M.; DA SILVA, EDMUNDO; RICCI, RITCHELLI; MARCIANO, FERNANDA R.; WEBSTER, THOMAS J.; VASCONCELLOS, LUANA M. R.; LOBO, ANDERSON O.. Electrospun ultrathin PBAT/nHAp fibers influenced the in vitro and in vivo osteogenesis and improved the mechanical properties of neoformed bone. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 155, p. 544-552, . (11/17877-7, 11/20345-7, 15/09697-0, 15/08523-8, 16/00575-1)
MACHADO-PAULA, M. M.; CORAT, M. A. F.; LANCELLOTTI, M.; MI, G.; MARCIANO, F. R.; VEGA, M. L.; HIDALGO, A. A.; WEBSTER, T. J.; LOBO, A. O.. A comparison between electrospinning and rotary-jet spinning to produce PCL fibers with low bacteria colonization. Materials Science & Engineering C-Materials for Biological Applications, v. 111, . (11/20345-7, 15/08523-8, 11/17877-7, 14/50869-6, 14/16295-2, 15/09697-0)
RODRIGUES, BRUNO V. M.; DIAS, VANESSA M.; FRAGA, MARIANA A.; DA SILVA SOBRINHO, ARGEMIRO S.; LOBO, ANDERSON O.; MACIEL, HOMERO S.; PESSOA, RODRIGO S.. Atomic layer deposition of TiO2 thin films on electrospun poly (butylene adipate-co-terephthalate) fibers: Freestanding TiO2 nanostructures via polymer carbonization. MATERIALS TODAY-PROCEEDINGS, v. 14, p. 7-pg., . (15/10876-6, 17/18826-3, 15/09697-0, 11/50773-0, 12/15857-1, 11/20345-7, 16/00575-1, 15/05956-0, 18/01265-1)
STOCCO, THIAGO D.; BASSOUS, NICOLE J.; ZHAO, SIQI; GRANATO, ALESSANDRO E. C.; WEBSTER, THOMAS J.; LOBO, ANDERSON O.. Nanofibrous scaffolds for biomedical applications. NANOSCALE, v. 10, n. 26, p. 12228-12255, . (15/09697-0)
STOCCO, T. D.; RODRIGUES, B. V. M.; MARCIANO, F. R.; LOBO, A. O.. Design of a novel electrospinning setup for the fabrication of biomimetic scaffolds for meniscus tissue engineering applications. Materials Letters, v. 196, p. 221-224, . (11/17877-7, 15/08523-8, 11/20345-7, 16/00575-1, 15/09697-0)
SILVA SOARES, LUIS EDUARDO; NAHORNY, SIDNEI; BRAGA, VIVIAN DE FARIA; MARCIANO, FERNANDA ROBERTA; BHATTACHARJEE, TANMOY T.; LOBO, ANDERSON OLIVEIRA. Raman spectroscopy-multivariate analysis related to morphological surface features on nanomaterials applied for dentin coverage. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, v. 228, . (11/20345-7, 11/17877-7, 16/00575-1, 13/11192-8, 14/21587-2, 15/09697-0, 14/02163-7)
NAHORNY, SIDNEI; ZANIN, HUDSON; CHRISTINO, VINIE ABREU; MARCIANO, FERNANDA ROBERTA; OLIVEIRA LOBO, ANDERSON; SILVA SOARES, LUIS EDUARDO. Multi-walled carbon nanotubes/graphene oxide hybrid and nanohydroxyapatite composite: A novel coating to prevent dentin erosion. Materials Science & Engineering C-Materials for Biological Applications, v. 79, p. 199-208, . (14/21587-2, 11/17877-7, 11/20345-7, 15/50093-0, 13/20825-4, 14/02163-7, 15/09697-0, 13/11192-8, 16/00575-1)
PESSOA, R. S.; DOS SANTOS, V. P.; CARDOSO, S. B.; DORIA, A. C. O. C.; FIGUEIRA, F. R.; RODRIGUES, B. V. M.; TESTONI, G. E.; FRAGA, M. A.; MARCIANO, F. R.; LOBO, A. O.; et al. TiO2 coatings via atomic layer deposition on polyurethane and polydimethylsiloxane substrates: Properties and effects on C. albicans growth and inactivation process. Applied Surface Science, v. 422, p. 73-84, . (11/50773-0, 12/15857-1, 11/17877-7, 11/20345-7, 15/10876-6, 15/09697-0, 15/08523-8, 16/00575-1, 15/05956-0)
SALLES, GEISA NOGUEIRA; DOS SANTOS PEREIRA, FERNANDA APARECIDA; PACHECO-SOARES, CRISTINA; MARCIANO, FERNANDA ROBERTA; HOLSCHER, CHRISTIAN; WEBSTER, THOMAS J.; LOBO, ANDERSON OLIVEIRA. A Novel Bioresorbable Device as a Controlled Release System for Protecting Cells from Oxidative Stress from Alzheimer's Disease. Molecular Neurobiology, v. 54, n. 9, p. 6827-6838, . (11/17877-7, 11/20345-7, 15/09697-0, 14/20561-0, 16/00575-1)

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