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Interference the fipronil insecticide on responses on oxidative stress of Tilapia Nile mediated by gamma-aminobutyric acid (GABA) during periods of hypoxia.

Grant number: 15/15191-1
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): November 01, 2015
Effective date (End): April 30, 2019
Field of knowledge:Biological Sciences - Biochemistry - Metabolism and Bioenergetics
Principal researcher:Claudia Regina Bonini Domingos
Grantee:Priscila Leocadia Rosa Dourado
Home Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil
Associated scholarship(s):17/18210-2 - Combined effects of the insecticide Fipronil (REGENT® 800WG) and climate change factors on the metabolism of the benthic fish Solea senegalensis, BE.EP.DR


Regent®800WG is an important pesticide widely used in sugar cane cultures in Brazil and contains the active ingredient fipronil. The main mechanism of action of fipronil is the inhibition of normal nervous impulse by competing with the ³-aminobutyric acid (GABA), important inhibitory neurotransmitter of the central nervous system. Some studies have indicated possible binding of fipronil to GABA receptors in vertebrates, promoting neurotoxic effects in exposed organisms. Fipronil is a persistent compound in soil and has been widely detected in aquatic environments. Adverse effects on the environment such as the condition of hypoxia, can lead to a significant increase in GABA levels, and the presence of fipronil in aquatic environments can influence the cerebral protection of the animal against the negative effects of hypoxia, promoting changes in physiological processes, which can cause oxidative stress in aquatic organisms. In this study, we propose to evaluate the influence of exposure to commercial formulation of fipronil (Regent®800WG) in oxidative stress, levels of GABA, in the expression of GABA (A) receptor in the brain and oxidative stress parameters in liver and gills of Oreochromis niloticus submitted to hypoxia. Fish will be exposed to different periods of hypoxia (1h, 3h and 8h) in absence of the contaminant to select the time of hypoxia in which biochemical responses were more evident. Then, the effects of fipronil (concentrations of 0.1 µg/L-1 and 0.5 µg/L-1 ) will be evaluated in combination to hypoxia effects. Oxidative stress biomarkers will be analysed by enzymes superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione redutase (GR) activity and lipid peroxidation. The biotransformation enzyme glutathione S-transferase (GST) activity, the quantification of protein carbonyls, as well the neurotransmitters levels, receptor expression GABA(A), hypoxia factor induction of expression (HiF-1±) and genotoxic effects also will be evaluated.Keywords: pesticide, environmental effects, neurotoxicity, biomarkers, fish

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Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
DOURADO, Priscila Leocadia Rosa. Interference the fipronil insecticide on responses on oxidative stress of Tilapia Nile mediated by gamma-aminobutyric acid (GABA) during periods of hypoxia.. 2019. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências Letras e Ciências Exatas. São José do Rio Preto São José do Rio Preto.

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