Characterization of gastrointestinal motility in diabetes mellitus experimental models: an approach using clamp hyperglycemic and hyperinsulinemic

Abstract

Diabetes mellitus is a worldwide public health problem and is often associated with symptoms and gastrointestinal motility disorders. Thus, the objective is to evaluate the profile of gastric motility in rats and compare with glycemic variations across the hyperinsulinemic and hyperglycemic clamp technique in experimental models of severe and moderate diabetes and gastrointestinal peptides. To determine the metabolism of insulin in the body will be adopted the hyperinsulinemic clamp and the hyperglycemic. The assessment of contractility and gastric emptying is performed by AC Biosusceptometria (BAC). The acquisition of electrical activity will be performed by electrogastrography (EGG). Performing the clamp techniques can analyze glucose changes in animals subjected to different models of diabetes, as well as to analyze over time as these conditions may be involved in changes in gastrointestinal motility. Will be used to determine the GLP1 and GIP incretins, as well as histological analysis of gastric tissue to assess how acute and chronic diabetes can functionally change the TGI. Among the studies focusing on diabetes mellitus, such characterization is unprecedented and may elucidate the electric log and mechanical as well as the pathophysiological changes of severe and moderate diabetes in TGI over time, glycemic conditions and their interrelations.