Fluoride (F) is an extremely important element in terms of public health due to its properties to prevent or reverse caries in individuals of all ages. It can offer risks to the body if consumed or applied indiscriminately or inappropriately, such as dental fluorosis. After its absorption, F is distributed through the blood stream and stored in calcified tissues and soft tissues, and its excretion is essentially via the kidney. Several molecular mechanisms involving the F metabolism are still unknown. In the last decade, many studies have sought to unravel the influence of genetic factors in genetic susceptibility to dental fluorosis using mouse strains that have very different responses to the effects F. Thus, there is evidence that resistance and susceptibility to fluorosis seem to be influenced by genetic factors as well as the interaction with the environment, such as physical activity, and diet. The current proposal is through proteomics analysis, identify proteins that are altered in kidney and muscle of mice susceptible or resistant to fluorosis in response to changes in exposure F, with and without exercise. Therefore, 60 animals of the strains will be obtained A / J (n = 30) and 129P3 / J (n = 30), susceptible and resistant to fluorosis, respectively. They animals will be divided into six groups according to each treatment, receiving the drinking water containing 0 or 50 ppm F, for 42 days. The groups with physical activity will be held daily races on a treadmill for 60 minutes. After the completion of the treatment, the animals will be euthanized, for collecting samples and conducting analyzes.
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