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Virulence gene expression of clinical isolates of Candida albicans treated with Antimicrobial Photodynamic Therapy

Grant number: 15/13409-0
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): October 01, 2015
Effective date (End): February 28, 2017
Field of knowledge:Health Sciences - Dentistry - Dental Clinics
Principal Investigator:Ana Cláudia Pavarina
Grantee:Gabriela Caroline Alonso
Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
Associated research grant:13/07276-1 - CEPOF - Optics and Photonic Research Center, AP.CEPID


The aim of this longitudinal study is to evaluate the virulence gene expression of clinical isolates of Candida albicans from patients with denture stomatitis after Photodynamic Therapy (PDT). For this reason, it will be used isolates of Candida albicans collected from a PhD research that is ongoing (FAPESP 2014/01262-1). In this referred study, 60 denture wearers with denture stomatitis will be randomly assigned to 2 groups (n = 30), according to established treatment : Group 1 (NYS) - patiets will be treated with topical antifungal nystatin (oral suspension), 4 times per day for 15 days, Group 2 (PDT) - the palate and denture will be treated with PDT, using Photodithazine (PDZ - 20 min) associated with LED light (660 nm) in a dose of 50 J/cm2, 3 times per week for 15 days. All patients will be followed for 60 days after initiating treatments. Collections of mycological material from dentures and palatal mucosa will be taken at baseline (day 0), at the end of the treatment (day 15) and at the follow-up time intervals (days 30, 45 ad 60). From the Candida albicans isolates, it will be quantified the expression of the virulence factors: capability of degradative enzymes secretion (proteinase and fosfolipase), lipase enzyme, hiphae formation ability and oxidative stress adaptation.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ALONSO, GABRIELA C.; PAVARINA, ANA C.; SOUSA, TABATA V.; KLEIN, MARLISE I.. A quest to find good primers for gene expression analysis of Candida albicans from clinical samples. Journal of Microbiological Methods, v. 147, p. 1-13, . (13/07276-1, 15/13409-0)
ALONSO, GABRIELA CAROLINE; KLEIN, MARLISE INEZ; JORDAO, CLAUDIA CAROLINA; CARMELLO, JULIANA CABRINI; PAVARINA, ANA CLAUDIA. Gene expression of Candida albicans strains isolates from patients with denture stomatitis submitted to treatments with photodynamic therapy and nystatin. Photodiagnosis and Photodynamic Therapy, v. 35, . (15/13409-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
ALONSO, Gabriela Caroline. Evaluation, design and standardization of primers for Candida albicans virulence genes and their expression in clinical isolates submitted to antifungal therapies. 2017. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araraquara Araraquara.

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