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Evaluation the effect of different commercial mouth rinses on the viability and activity of microcosm biofilm activity and on enamel demineralization

Grant number: 15/11635-2
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): September 01, 2015
Effective date (End): July 31, 2017
Field of knowledge:Health Sciences - Dentistry - Social and Preventive Dentistry
Principal Investigator:Ana Carolina Magalhães
Grantee:Aline Silva Braga
Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil


Many antimicrobials are used to control dental biofilm; among them the most studied agent is chlorhexidine. Commercial mouth rinses are often used by the population, without prescription, aiming to control halitosis. Chlorhexidine, cetylpyridinium chloride, xylitol, fluoride, triclosan and essential oils are examples of active agents included into commercial mouth rinses; however, there are few comparative studies. This work will evaluate the effect of these commercial mouth rinses on the viability and activity of a microcosm biofilm as well as on the prevention of enamel demineralization. For the formation of a microcosm biofilm, saliva will be collected from 2 healthy subjects, who have not brushed their teeth in the last 24 hours and have not ingested food or drinking liquids in the last 2 hours. Saliva will be diluted (70% saliva and 30% glycerol) and aliquots of 1 ml of saliva will be stored at -80° C and further mixture to a Mc Bain saliva (with 0.2% sucrose) for the formation of a cariogenic biofilm. Bovine enamel samples (4 mm x 4 mm) will be prepared for the formation of a microcosm biofilm for 14 days, which will be treated daily (1x60s / day) using the following commercial mouth rinses: Oral-B Complete, Listerine, Cepacol, Periogard, Noplak Max and Malvatricin Plus. In Step 1, live and dead bacteria in the biofilm will be evidenced by fluorescence using confocal microscope. In Step 2, the biofilm acidogenicity will be assessed, by measuring the presence of lactic acid using enzymatic methods. In Step 3, the microbial suspension removed from the biofilm will be diluted and spread on agar plates for counting the colony forming units (CFU) for total microorganisms, total Streptococcus and Streptococcus mutans. In Step 4, the demineralization caused by biofilm in the studied conditions will be measured by transverse microradiography. The data will be subjected to appropriate statistical analysis (parametric or non-parametric test, p <0.05). (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
BRAGA, Aline Silva. Evaluation of the effect of different commercial mouthrinses on the viability and activity of microcosm biofilm and on enamel demineralization. 2017. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Odontologia de Bauru (FOB/SDB) Bauru.

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