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Effect of the activation of protease activated receptor type 1 (PAR1)on the expression of osteogenic factors in periodontal ligament cells

Grant number: 15/11587-8
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): October 01, 2015
Effective date (End): May 31, 2018
Field of knowledge:Health Sciences - Dentistry - Periodontology
Principal Investigator:Marinella Holzhausen Caldeira
Grantee:Emanuel da Silva Rovai
Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil


The activation of the protease activation receptor type 1 by thrombin may play an important role in homeostasis and repair of the periodontal tissues. A recent study from our group demonstrated an increased PAR1 expression in gingival fluid cells in patients with chronic periodontitis after periodontal treatment, and the PAR1 expression was associated with a decreased expression of inflammatory biomarkers. In addition, in vitro studies have shown that the activation of PAR1 by thrombin induces the synthesis of osteoprotegerin (OPG) in periodontal ligament cells, leads to a dose-dependent increase proliferation of cells isolated from bone marrow stroma of rats and induces the expression of growth factors in osteoblasts. Therefore, studies suggests an important role of PAR1 in the periodontal tissue repair. In this study, we hypothesized that activation of PAR1 in the periodontal ligament cells can lead to increased osteogenic activity. Thus, the main objective of this study is to evaluate the effect of PAR1 activation by thrombin on the osteogenic differentiation of ligament cells. Furthermore, will be evaluated the effects of the PAR1 activation on gene and protein expression of alkaline phosphatase (ALP), osteopontin, osteocalcin, RANKL, OPG and runt-related transcription factor-2 (Runx2).

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ROVAI, EMANUEL SILVA; BATITUCCI AMBROSIO, LUCAS MACEDO; DE FRANCA, BRUNO NUNES; DE OLIVEIRA, LETICIA RODRIGUES; GASPARONI, LETICIA MIQUELITTO; SIPERT, CARLA RENATA; HOLZHAUSEN, MARINELLA. Protease-Activated Receptor Type 1 Activation Enhances Osteogenic Activity in Human Periodontal Ligament Stem Cells. STEM CELLS INTERNATIONAL, . (15/07396-2, 15/23746-3, 15/11587-8, 16/11399-0)
BRUNO NUNES DE FRANÇA; LETÍCIA MIQUELITTO GASPARONI; EMANUEL SILVA ROVAI; LUCAS MACEDO BATITUCCI AMBRÓSIO; NATHALIA FELIX DE MENDONÇA; MARCOS HIDEKI HAGY; ALDRIN HUAMÁN MENDOZA; CARLA RENATA SIPERT; MARINELLA HOLZHAUSEN. Protease-activated receptor type 2 activation downregulates osteogenesis in periodontal ligament stem cells. Brazilian Oral Research, v. 37, . (15/11587-8, 15/07396-2, 16/11399-0)
RAHHAL, JULIANA GARUBA; ROVAI, EMANUEL DA SILVA; HOLZHAUSEN, MARINELLA; CALDEIRA, CELSO LUIZ; DOS SANTOS, CARLOS FERREIRA; SIPERT, CARLA RENATA. Root canal dressings for revascularization influence in vitro mineralization of apical papilla cells. Journal of Applied Oral Science, v. 27, . (15/09750-8, 15/03965-2, 16/13944-5, 15/11587-8)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
ROVAI, Emanuel da Silva. Effect of protease activated receptor type 1 (PAR1) activation on the osteogenic activity of periodontal ligament cells. 2018. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Odontologia (FO/SDO) São Paulo.

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