Advanced search
Start date
Betweenand

Occurrence and molecular characterization of Cryptosporidium spp. in Brazilian wildlife deer

Grant number: 15/10086-5
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): August 01, 2015
Effective date (End): July 31, 2017
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal researcher:Katia Denise Saraiva Bresciani
Grantee:Weslen Fabricio Pires Teixeira
Home Institution: Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

Cryptosporidiosis is a severe enteric disease, responsible for intestinal disorders, especially in young ruminants, with varied clinical manifestations and eventual mortality. The present research project has the goal of evaluating occurrence, as well as genetically characterizing, Cryptosporidium infections in Brazilian wildlife deer. Presence of such protozoan's oocysts in cervid fecal samples will be observed by negative staining with malachite green and phase contrast microscopy in Sheather solution. Genomic DNA of each fecal sample will be extracted, allowing amplification of the parasite's DNA (gene 18S RNA and GP 60) by means of polymerase chain reaction (nested-PCR). Positive samples regarding Cryptosporidium spp. DNA will be sequenced, enabling molecular characterization of this coccidian. Statistical analysis will consist of the Chi-Square test, to verify the association of the aforementioned enteroparasite related to the origin of fecal samples. (AU)

News published in Agência FAPESP Newsletter about the scholarship:
Articles published in other media outlets (0 total):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PIRES TEIXEIRA, WESLEN FABRICIO; DE OLIVEIRA, MARCIO LEITE; DE FARIA PERES, PEDRO HENRIQUE; NAGATA, WALTER BERTEQUINI; SANTANA, BRUNA NICOLETI; MIRANDA OLIVEIRA, BRUNO CESAR; BARBANTI DUARTE, JOSE MAURICIO; CARDOSO, TEREZA CRISTINA; ZANETTI LOPES, WELBER DANIEL; SARAIVA BRESCIANI, KATIA DENISE. Cryptosporidium parvum in brown brocket (Mazama gouazoubira) from Brazil: First report of the subtype IIaA16G3R1 in cervids. Parasitology International, v. 80, . (10/50748-3, 15/10086-5, 15/25742-5, 12/50206-1)
PIRES TEIXEIRA, WESLEN FABRICIO; DE OLIVEIRA, MARCIO LEITE; DE FARIA PERES, PEDRO HENRIQUE; MIRANDA OLIVEIRA, BRUNO CESAR; NAGATA, WALTER BERTEQUINI; VIEIRA, DIELSON DA SILVA; DE ANDRADE JUNIOR, ANASSILTON MOREIRA; FERRARI, ELIS DOMINGOS; BARBANTI DUARTE, JOSE MAURICIO; MEIRELES, MARCELO VASCONCELOS; et al. IRST REPORT OF GENUS Cryptosporidium IN CERVIDS SPECIES: Mazama americana, Mazama nana AND Blastocerus dichotomu. VETERINARY RESEARCH COMMUNICATIONS, v. 46, n. 1, . (10/50748-3, 15/10086-5, 12/50206-1, 15/25742-5)

Please report errors in scientific publications list by writing to: cdi@fapesp.br.