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New ceramic materials: evaluation of biofilm formation and viability of human gingival fibroblasts (FMM-1)

Grant number: 15/13322-1
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): September 01, 2015
Effective date (End): January 31, 2017
Field of knowledge:Health Sciences - Dentistry - Dental Materials
Principal Investigator:Marco Antonio Bottino
Grantee:Amanda Maria de Oliveira Dal Piva
Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil
Associated scholarship(s):16/06272-0 - Fatigue strength of zirconia-reinforced lithium silicate ceramic under different loading conditions, BE.EP.MS


This study aims to evaluate the surface roughness of two new restorative materials and the formation of monotypic and heterotypic biofilms, as well as the viability of human gingival fibroblasts (FMM-1) in contact with these materials. For this, 63 blocks (4.5x4.5x1.5 mm) of each ceramic (high translucency zirconia partially stabilized by yttrium and lithium silicate reinforced by zirconium) will be made. The blocks will be standardized after polishing and will receive a glaze application. The surface roughness (Ra, Rz, RSm) will be evaluated through a profilometer and surface free energy will be determined by a goniometer. After sterilization, specimens will be contaminated for monotypic biofilm formation of S. mutans (ATCC 35688), S. sanguinis (ATCC 7073) and C. albicans (ATCC 18804) and for heterotypic biofilms with these microorganisms during 16 h formation. The biofilm formed on the materials will be quantified by counting the colony forming units (CFU/mL) and flow cytometry, and the viability of human gingival fibroblasts to the materials will be evaluated by MTT assay. Qualitative analysis of specimens will be performed by scanning electron microscopy, atomic force microscopy and confocal laser scanning microscopy. The results will be statistically analyzed and discussed against the literature. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DAL PIVA, A. M. O.; CONTRERAS, L. P. C.; RIBEIRO, F. C.; ANAMI, L. C.; CAMARGO, S. E. A.; JORGE, A. O. C.; BOTTINO, M. A.. Monolithic Ceramics: Effect of Finishing Techniques on Surface Properties, Bacterial Adhesion and Cell Viability. Operative Dentistry, v. 43, n. 3, p. 315-325, . (14/19357-9, 15/13322-1)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
PIVA, Amanda Maria de Oliveira Dal. In vitro biofilm formation and viability of human gingival fibroblasts (FMM-1) on new ceramic materials. 2017. Master's Dissertation - Universidade Estadual Paulista (Unesp). Instituto de Ciência e Tecnologia. São José dos Campos São José dos Campos.

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