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Terpinen-4-ol: study of synergistic/additive effect, adhesion in co-culture and change of virulence factors of Candida spp

Grant number: 15/00155-0
Support type:Scholarships in Brazil - Master
Effective date (Start): August 01, 2015
Effective date (End): March 31, 2016
Field of knowledge:Health Sciences - Dentistry
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal researcher:Denise Madalena Palomari Spolidorio
Grantee:Caroline Coradi Tonon
Home Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil


The genus Candida can be found in up to 50% of healthy individuals causing no apparent damage, but under predisposing conditions such as systemic diseases or physiological conditions, can become pathogenic causing inflammation and tissue destruction. Candida spp. in the form of biofilms are important in the development of infections because they are associated with high levels of resistance to antimicrobial agents. Alternative therapies with natural extracts open new perspectives for prevention and control of oral diseases in the search for favorable therapeutic effects. The Terpinen-4-ol is a monoterpene engaged in the membrane loss of induction and presents broad spectrum of antimicrobial activity and anti-inflammatory activity. Therefore, the objective of this study is to evaluate the antifungal, synergistic/additive, adhesion and changes in the virulence factors of Terpinen-4-ol on Candida albicans and Candida tropicalis. Thus, the identification of the MIC will be performed (Minimum Inhibitory Concentration) and MFC (Minimum Fungicidal Concentration) of Terpinen-4-ol on standard strains of C. albicans(ATCC 90028) and C. tropicalis(ATCC4563), using the method broth microdilution. Simple and multispecies biofilms will be prepared using the static microtiter plate model and quantified by colony forming units (CFU/mL). The effect of Terpinen-4-ol in the adhesion ofC. albicansand C. tropicaliswill be held in co-culture with oral NOK cells as well as virulence factors (proteases, phospholipases andhemolysin) will be evaluated in vitro. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CAROLINE CORADI TONON; RENATA SERIGNOLI FRANCISCONI; ESTER ALVES FERREIRA BORDINI; PATRÍCIA MILAGROS MAQUERA HUACHO; JANAÍNA DE CÁSSIA ORLANDI SARDI; DENISE MADALENA PALOMARI SPOLIDORIO. Interactions between Terpinen-4-ol and Nystatin on biofilm of Candida albicans and Candida tropicalis. Brazilian Dental Journal, v. 29, n. 4, p. 359-367, . (15/00155-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
TONON, Caroline Coradi. Terpinen-4-ol: study of synergistic / additive effect, adhesion in co-culture and the modification of virulence factors of Candida spp. 2016. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araraquara Araraquara.

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