There are an increasing number of infections caused by Gram-negative bacteria producing extended spectrum betalactamases, and the emergence of new therapeutic resources does not follow the evolution of resistance mechanisms, making them a serious public health problem. The extended spectrum ²-lactamases (ESBL) are enzymes that hydrolyze the ²-lactam ring of penicillins, cephalosporins and aztreonam, conferring resistance to these antimicrobials. Brazil is the largest exporter of chicken means that the presence of ESBL and it becomes also a potential risk in the agribusiness sector. Based on this, the present project aims to identifying some groups of resistance genes and the plasmids encoding these genes of ESBL production in Enterobacteriaceae isolated from Brazilians chicken meat, chicken feaces and human feaces. The molecular detections will be by PCR and sequencing. Furthermore, the epidemiology profile of each sample will be featured by the sequencing of genes "housekeeping" (MLST) to clonal characterization of them. The results of this study will be essential to understanding the ESBL resistance genes profiles most commonly found in the chain of the broilers production in the state of São Paulo, and also to show the correlation between the presence of resistance genes in animals and humans.
News published in Agência FAPESP Newsletter about the scholarship: