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Fluorescent probe development for specific determination of halogenating activity of enzymes myeloperoxidase and eosinophil peroxidase

Grant number: 15/08267-1
Support type:Scholarships in Brazil - Master
Effective date (Start): July 01, 2015
Effective date (End): February 28, 2017
Field of knowledge:Biological Sciences - Biochemistry - Enzymology
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal researcher:Valdecir Farias Ximenes
Grantee:Luiza de Carvalho Bertozo
Home Institution: Faculdade de Ciências (FC). Universidade Estadual Paulista (UNESP). Campus de Bauru. Bauru , SP, Brazil

Abstract

Peroxidases are enzymes which catalyze the oxidation of different substrates from the reduction of hydrogen peroxide. The peroxidases present in leukocytes, myeloperoxidase (MPO) and eosinophil peroxidase (EPO), are characterized by their exclusive catalytic capacity in the oxidation of chloride and bromide to hypochlorous acid (HOCl) and hypobromous (HOBr), respectively. These oxidants are characterized by their intense reactivity and implications in several deleterious processes, which are well established and accepted by the scientific community. The determination of the enzymatic activity of these enzymes is not so trivial, because in addition to acting through its halogenating cycle (production HOCl and HOBr), these enzymes also act on various substrates through its peroxidase cycle. Thus, the available methods for the determination the halogenating activity are often indirect and/or of low selective. Here, based on a recent discovery of our research group, we intend to develop a direct and selective method for the determination of halogenating activity of these enzymes. Our proposal is the application of a fluorescent probe (dansylglycine), which reacts readily with HOBr, due to its electrophilic character, but is not reactive with others oxidants. Thus, our hypothesis is that we could correlate the halogenating activity of these enzymes with the change in fluorescence of this probe and, consequently, to propose a new analytical technique for those who need to study processes in which these enzymes are involved. (AU)

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Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
BERTOZO, Luiza de Carvalho. Development of fluorescent probe for specific determination of halogenating activity of enzymes myeloperoxidase and eosinophil peroxidase. 2017. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Ciências. Bauru Bauru.

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