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Oxidative stress evaluation in rats with rheumatoid arthritis after gold ferromagnetic nanoparticles administration

Grant number: 15/05383-0
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): June 01, 2015
Effective date (End): January 31, 2016
Field of knowledge:Health Sciences - Pharmacy - Toxicological Analysis
Principal Investigator:Maria Fernanda Hornos Carneiro
Grantee:Ana Flavia do Bem Afonso
Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Rheumatoid arthritis (RA) is a systemic, autoimmune and incurable chronic inflammatory disease affecting synovial tissues causing pain, stiffness and inflammation. Medications containing metal gold (Au) have shown good results in improving symptoms of RA. However, due to the high toxicity of the metal and its wide tissue distribution, several side effects are observed during treatment. More recently, nanomaterials have been gaining significant attention, mainly for their physicochemical characteristics that confer more potent action and consequent reduction of dose, in addition to its potential use allied to intelligent systems - that can both control the release of nanomaterials and/or direct them to the target. In this proposal, we intend to evaluate the potential toxicity of Au nanoparticles linked to superparamagnetic iron oxide nanoparticles (SPIONs) - in mice with collagen-induced AR. For this purpose, adult male Wistar rats will be divided into the following treatment groups (n = 6/group): 1) negative controls (without inducing AR), 2) positive controls (induction with RA, treatment with SPIONS without Au) 3) SPIONs containing Au, 4) colloidal Au nanoparticles and 5) Methotrexate (0.1 mg / kg). In order to verify toxicity oxidative stress parameters such as levels of F2-isoprostanes, total glutathione, glutathione peroxidase and catalase activities will be determined. As there will be magnetic orientation of the therapeutic agent to the affected region, it is expected to find minimized side and toxic effects frequently found during the conventional therapeutic procedure for AR (methotrexate) which is relevant and will be assessed by the oxidative stress parameters this project aim to evaluate.

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