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Incorporation of epigallocatechin gallate in etch-and-rinse adhesive system and its effect on flexural strength and bond durability to caries-affected dentin

Grant number: 15/06259-1
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): August 01, 2015
Effective date (End): July 31, 2016
Field of knowledge:Health Sciences - Dentistry - Dental Clinics
Principal Investigator:Flavia Lucisano Botelho Do Amaral
Grantee:Renata Czech
Host Institution: Centro de Pesquisas Odontológicas São Leopoldo Mandic. Faculdade São Leopoldo Mandic (SLMANDIC). Sociedade Regional de Ensino e Saúde S/S Ltda (SRES). Campinas , SP, Brazil


The present in vitro study aims to evaluate the incorporation of epigallocatechin gallate (EGCG), a metalloproteinase-MMP inhibitor, in etch-and-rinse adhesive system and its effect on flexural resistance (FR) and bond durability to caries-affected dentin, by means of microtensile bond strength testing (MBS). For MBS, forty dentin slabs will be submitted to a microbiological caries model to induce caries formation. After infected caries removal, caries affected dentin surface will be acid conditioned (37% phosphoric acid) for 15 seconds and randomly divided into four groups according to the application mode of the MMP-inhibitory solution on dentin surface (n=10): EGCG-PRE - EGCG solution (200¼g/mL) applied before adhesive system (as a pre-treatment); EGCG-INC - EGCG solution incorporated to the etch-and-rinse adhesive system at a ratio of 200¼g/mL; CLX-PRE - 2% chlorhexidine digluconate solution applied before adhesive system (as a pre-treatment) (positive control); NT - Any treatment will be performed (negative control). Adhesive system will be applied according to manufacture's instruction and composite restorative will be placed on dentin surface. Dentin-resin blocks will be sectioned into 8 beam-shaped specimens (0.8mm2 area) that will be allocated into two groups, according to the moment of microtensile bond strength evaluation: 24 hours or after a 6-months period of water storage. For FR analysis, bar-shaped specimens (7mm x 2mm x 1mm - length x width x thickness) will be obtained with the two-step adhesive system, containing or not the EGCG solution (200¼g/mL). Both MBS and FR tests will be performed in a universal testing machine, operating at a 0.5 mm/min speed. Data will be statistically analyzed considering its distribution. If there is normality of errors distribution and homogeneity of variance, parametric tests will be applied; on the contrary, non-parametric testes will be adopted, both with 5% significance level.

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(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CZECH, R.; OLIVEIRA, C. A. R.; FRANCA, F. M. G.; BASTING, R. T.; TURSSI, C. P.; AMARAL, F. L. B.. Incorporation of EGCG into an etch-and-rinse adhesive system: mechanical properties and bond strength to caries affected dentin. JOURNAL OF ADHESION SCIENCE AND TECHNOLOGY, v. 33, n. 22, . (15/06259-1)

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