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Evaluation of microbial diversity of EGSB reactor on a pilot scale for the removal of anionic surfactant in wastewater commercial laundry diluited in sewage

Grant number: 15/08219-7
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): July 01, 2015
Effective date (End): December 31, 2015
Field of knowledge:Engineering - Sanitary Engineering - Water Supply and Wastewater Treatment
Principal Investigator:Tiago Palladino Delforno
Grantee:Victor Borin Centurion Biruel
Host Institution: Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas (CPQBA). Universidade Estadual de Campinas (UNICAMP). Paulínia , SP, Brazil


The project objective is to act in a synergistic and complementary way with a work in progress in Biological Processes Laboratory (LPB / USP-São Carlos), through the evaluation of microbial diversity using independent growing techniques (PCR / DGGE and sequencing of the gene 16S rRNA). Therefore, the samples will be derived from a biological reactor EGSB (expanded granular sludge bed) applied for the treatment of anionic surfactant (linear alkyl benzene sulfonate) in wastewater diluted commercial laundry in domestic sewage. It is noteworthy that reactor is in operation phase (LPB / USP-São Carlos) by graduation student Alana Gandra L. de Moura oriented by Prof. Dr. Maria Bernadette A. Varesche. Four objectives will be evaluated in this project: (I) diversity of Archaea and Bacteria Domain in the reactor samples; (II) evaluation of possible microbial stratification along the reactor, (III) to assess the diversity of the key gene (bamA) involved in anaerobic degradation of aromatic compounds and (IV) characterize the phylogenetically of microbiological group Bacteria. This study will contribute to the EGSB reactor operating data, allowing deepen understanding of anaerobic removal of LAS in biological reactor. It is emphasized that integration of different areas (Process Engineering - with the use of biological reactors and Microbiology) aims to improve the process and the knowledge of the relationships between the members of the microbial consortium responsible for the degradation of LAS molecule.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CENTURION, V. B.; MOURA, A. G. L.; DELFORNO, T. P.; OKADA, D. Y.; DOS SANTOS, V. P.; VARESCHE, M. B. A.; OLIVEIRA, V. M.. Anaerobic co-digestion of commercial laundry wastewater and domestic sewage in a pilot-scale EGSB reactor: The influence of surfactant concentration on microbial diversity. INTERNATIONAL BIODETERIORATION & BIODEGRADATION, v. 127, p. 77-86, . (15/06246-7, 16/11948-3, 15/08219-7, 14/16426-0)

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