Advanced search
Start date

Assessment of neutrophil extracellular traps formation in CD40 ligand deficient patients and influence of in vitro treatment with interferon-gamma

Grant number: 15/01699-3
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): June 01, 2015
Effective date (End): March 31, 2017
Field of knowledge:Biological Sciences - Immunology
Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Antonio Condino Neto
Grantee:Tábata Takahashi França
Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil


Neutrophils are the most abundant cell type in circulation and constitute the first line of defense against pathogens in infection sites. They mediate inflammatory reactions and eliminate microorganisms through multiple mechanisms, such as the release of antimicrobial factors into phagosomes and in the extracellular medium. Recent studies demonstrated another type of neutrophil response against pathogens, the neutrophil extracellular traps, or NETs. NETs are composed by granular contents, as elastase and myeloperoxidase, and decondensed chromatin, forming a structure able to annul virulence factors and kill extracellular microorganisms. The Hyper-IgM syndrome X-linked (X-HIGM) is a primary immunodeficiency (PID) caused by mutations in CD40 ligand (CD40L) expressed by CD4+ T cells characterized by elevated serum levels of IgM. In addition to the intra and extracellular bacterial infection, patients with X-HIGM are susceptible to fungal infections, particularly for Pneumocystis jiroveci, Aspergillus sp, Candida albicans and Paracoccidioides brasiliensis. Once evidences indicate that CD40-CD40L interactions influence in the phagocyte oxidative burst and the production of reactive oxygen species (ROS) was reported as an essential factor to NETs formation, we propose to investigate if the increased susceptibility to infections, especially fungal, in X-HIGM patients may be related to failure in the induction and formation of NETs and check the effect of treatment with IFN-³ in vitro. These studies open new perspectives for the development of therapeutic strategies in the field of PID. (AU)

News published in Agência FAPESP Newsletter about the scholarship:
Articles published in other media outlets (0 total):
More itemsLess items

Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
FRANÇA, Tábata Takahashi. Evaluation of NET formation in CD40 ligand deficient patients.. 2017. Master's Dissertation - Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) São Paulo.

Please report errors in scientific publications list by writing to: