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Analysis of the bacterial and the antimicrobial susceptibility of selected bacteria against antimicrobial agentes

Grant number: 14/27366-8
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): June 01, 2015
Effective date (End): February 28, 2017
Field of knowledge:Health Sciences - Dentistry - Dental Clinics
Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Brenda Paula Figueiredo de Almeida Gomes
Grantee:Augusto Rodrigues Lima
Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
Associated scholarship(s):16/18512-6 - Detection of Streptococcus mutans and glycoprotein Cnm from infected root canals and their associated dental abscesses, BE.EP.MS


Teeth with pulp necrosis presenting symptomatology and periapical bone destruction tend to be colonized by a more complex anaerobic microbiota compared to asymptomatic cases. Black pigment bacteria (BPB) including Prevotella and Porphyromonas species; Parvimonas and Fusobacterium spp. have been frequently isolated/ detected in symptomatic cases. However, due Thus, the aims of this study are: a) to investigate the composition of the microbiota of symptomatic (ie the presence of periapical abscess) and asymptomatic teeth with pulp necrosis by checkerboard; b) to determine the antimicrobial susceptibility the BPB, Parvimonas and Fusobacterium spp in front of the most commonly antimicrobials used in the dental clinics and c) to correlate the clinical findings with microbial/endotoxic date. Patients in need of endodontic therapy due to pulp necrotic tissues, with the presence of symptomatology (n = 10) as well as no symptoms (n = 10), will be selected. Samples for microbiological analysis will be collected, as well as from the dental abscess (n = 10). Part of the samples will be processed by checkerboard, and another part will be diluted, plated and incubated for further isolation and identification by genetic sequencing of BPB, Parvimonas and Fusobacterium spp that have grown on the plates. The antimicrobial susceptibility of BPB, Parvimonas and Fusobacterium spp will be determined by the E-test method using antibiotics: benzylpenicillin, amoxicillin, amoxicillin + clavulanic acid, erythromycin, azithromycin, clindamycin and metronidazole. The data will be tabulated and statistically analyzed. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
LIMA, AUGUSTO RODRIGUES; HERRERA, DANIEL RODRIGO; FRANCISCO, PRISCILA AMANDA; PEREIRA, ANDREA CARDOSO; LEMOS, JOSE; ABRANCHES, JACQUELINE; GOMES, BRENDA P. F. A.. Detection of Streptococcus mutans in symptomatic and asymptomatic infected root canals. CLINICAL ORAL INVESTIGATIONS, v. 25, n. 6, . (14/27366-8, 16/23950-2, 16/18512-6)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
LIMA, Augusto Rodrigues. Analysis of the bacterial content of symptomatic and asymptomatic primary endodontic infections and susceptibility os specific bacteria to antimicrobial agents. 2017. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba Piracicaba, SP.

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