Analysis of the bacterial and the antimicrobial susceptibility of selected bacteria against antimicrobial agentes

Abstract

Teeth with pulp necrosis presenting symptomatology and periapical bone destruction tend to be colonized by a more complex anaerobic microbiota compared to asymptomatic cases. Black pigment bacteria (BPB) including Prevotella and Porphyromonas species; Parvimonas and Fusobacterium spp. have been frequently isolated/ detected in symptomatic cases. However, due Thus, the aims of this study are: a) to investigate the composition of the microbiota of symptomatic (ie the presence of periapical abscess) and asymptomatic teeth with pulp necrosis by checkerboard; b) to determine the antimicrobial susceptibility the BPB, Parvimonas and Fusobacterium spp in front of the most commonly antimicrobials used in the dental clinics and c) to correlate the clinical findings with microbial/endotoxic date. Patients in need of endodontic therapy due to pulp necrotic tissues, with the presence of symptomatology (n = 10) as well as no symptoms (n = 10), will be selected. Samples for microbiological analysis will be collected, as well as from the dental abscess (n = 10). Part of the samples will be processed by checkerboard, and another part will be diluted, plated and incubated for further isolation and identification by genetic sequencing of BPB, Parvimonas and Fusobacterium spp that have grown on the plates. The antimicrobial susceptibility of BPB, Parvimonas and Fusobacterium spp will be determined by the E-test method using antibiotics: benzylpenicillin, amoxicillin, amoxicillin + clavulanic acid, erythromycin, azithromycin, clindamycin and metronidazole. The data will be tabulated and statistically analyzed. (AU)