Advanced search
Start date

Antioxidant and anti-inflammatory activities of menthol in skin wound healing

Grant number: 15/06636-0
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): June 01, 2015
Effective date (End): May 31, 2016
Field of knowledge:Biological Sciences - Pharmacology - General Pharmacology
Principal Investigator:Claudia Helena Pellizzon
Grantee:Eduardo Oliveira de Souza
Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil


The wound healing has been reasons to working over the years and the search for topical treatments to help in this process generated new pharmacotherapy, with large exploration of the animal products. The scar process steps described by authors are joined and receiving in general terms the following designation: inflammation, proliferation and maturation. Menthol is a terpene found in essential oils extracted from plants of the genus Mentha. This aim of this project is to evaluate the effect of menthol-based cream in skin wound healing, elucidating the mechanism of action. Male Wistar rats (n=8) will be randomly divided in five groups: cream base (control group), collagenase (positive control), and menthol-based cream at 0,25%, 0,5% or 1%. The animals will be anesthetized, and subsequently wounds in the dorsal region will be developed with the aid of a punch. Every day, the wounds will be treated, photographed and measured. The rats will be euthanized after 3, 7 or 14 days of treatment (in agreement with the phases which are described in the literature). Menthol antioxidant activity will be assessed in the skin samples, through ELISA method, by measuring the level of glutathione (GSH), and the activity of glutathione peroxidase (GPx) and reductase (GR), superoxide dismutase (SOD) and myeloperoxidase (MPO). The anti-inflammatory activity of menthol will be analyzed in the blood plasma, through ELISA's kits of cytokines TNF-±, IL-6 and IL-10. This project is linked on the post-doctoral project of the Doctor Ariane Leite Rozza (Fapesp process 2013/22202-4) and the regular support by Fapesp 2013/23340-1.

News published in Agência FAPESP Newsletter about the scholarship:
Articles published in other media outlets (0 total):
More itemsLess items

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ROZZA, ARIANE LEITE; BESERRA, FERNANDO PEREIRA; VIEIRA, ANA JULIA; OLIVEIRA DE SOUZA, EDUARDO; HUSSNI, CARLOS ALBERTO; MARTINEZ, EMANUEL RICARDO MONTEIRO; NOBREGA, RAFAEL HENRIQUE; PELLIZZON, CLAUDIA HELENA. The Use of Menthol in Skin Wound Healing-Anti-Inflammatory Potential, Antioxidant Defense System Stimulation and Increased Epithelialization. PHARMACEUTICS, v. 13, n. 11, . (13/23340-1, 13/22202-4, 15/06636-0, 16/13307-5)

Please report errors in scientific publications list by writing to: