Chemical study of Sinningia (Gesneriaceae) and evaluation of photoinduzed biologycal effect for PDT

Abstract

The use of natural products dates back to ancient times and many are known to treat various diseases and is an inspiration to medicinal chemistry and a valuable source for the production of drugs due to the enormous diversity of chemical classes of substances and distinct functions. Worldwide, and particularly in Brazil, holder of a fantastic biodiversity, only a small fraction of these organisms is explored. With the improvement of analytical techniques such as Nuclear Magnetic Resonance (NMR), Mass Spectrometry (MS) and metabolomics approaches and dereplication, for distinguishing novel molecules of already described, the number of organisms studied has increased, especially for plants. Thus, the objective of this project is to study the metabolomic profile of plant species of Gesneriaceae family, which features plants that are important in terms of biological activity and chemical diversity, gender Sinningia (Sinningia schiffneri Fritsch and Sinningia mauroana Chautems). For this use will be MS procedures, NMR or chromatography, applying dereplication approaches, with a view to the isolation of substances from bioactive extracts and the evaluation of the biological effect in photoinduced cell lines for use in Photodynamic Therapy (PDT). In the first stage of the study, chemical profiles will be obtained in order to identify some components for dereplication directly in crude extracts and in the second step, the isolation and chromatographic procedures employing structural identification using spectroscopic analysis (UV, IR, NMR (1D and 2D) and MS) in phytochemical study guided by absorption in the 400 to 800 nm region, the photochemical efficiency and the bioassays to evaluate the photoinduced biological effect in cell lines for use in Photodynamic Therapy (PDT). For fractionation the phytochemical study, from dereplication study, the evaluation of absorption in the UV-visible, the evaluation of photochemical efficiency and bioactivity, plant species will be chosen and the most promising extract with amount of mass to to continue the study to the isolation of substances capable of triggering photoinduced biological effect. This is an issue not much explored in the national scene and, although few literature data on photoactive molecules for use in PDT obtained from Brazilian species, these molecules and phyto-derived can be valuable because of the potential applications of PDT as therapeutic resource. (AU)